The distribution and organization of Ty1-copia-like retrotransposable elements in the genome of Vigna unguiculata (L) Walp (cowpea) and its relatives

被引:20
作者
Galasso, I [1 ]
Harrison, GE [1 ]
Pignone, D [1 ]
Brandes, A [1 ]
HeslopHarrison, JS [1 ]
机构
[1] JOHN INNES CTR PLANT SCI RES,DEPT CELL BIOL,KARYOBIOL GRP,NORWICH NR4 7UH,NORFOLK,ENGLAND
基金
英国生物技术与生命科学研究理事会;
关键词
Vigna unguiculata; cowpea; retrotransposons; genome organization; in situ hybridization; molecular evolution;
D O I
10.1006/anbo.1997.0443
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Ty1-copia-like retrotransposable elements were amplified from Vigna species by the polymerase chain reaction (PCR) with primers derived from the conserved region of the reverse transcriptase (RT) gene. Southern blots of genomic DNA digests from Vigna unguiculata and related species probed with the PCR derived sequences from V. unguiculata (rt-cow), V. unguiculata subsp. dekindtiana (rt-dek), V. luteola (rt-lut) and V. vexillata (rt-vex) showed variable hybridization patterns and intensities, generally correlating with taxonomic relationships. Sequencing of four clones revealed homologies to the reverse transcriptase gene of known Ty1-copia-like elements, although comparison of the predicted peptide sequences with each other and previously isolated reverse transcriptase genes from other legumes typically showed less than 50% identity. Fluorescence in situ hybridization of the PCR products showed that Ty1-copia elements represented a major fraction of the genome and were dispersed relatively uniformly over all chromosomes, with exclusion from centromeric, subtelomeric and nucleolar organizing regions, and a few sites of greater abundance. We are now able to start to understand the organization, variability and evolution of retrotransposons within the Fabaceae. (C) 1997 Annals of Botany Company.
引用
收藏
页码:327 / 333
页数:7
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