Screening of environmental DNA libraries for the presence of genes conferring lipolytic activity on Escherichia coli

被引：188

作者：

Henne, A

Schmitz, RA

Bömeke, M

Gottschalk, G

Daniel, R

机构：

[1] Univ Gottingen, Inst Mikrobiol & Genet, D-37077 Gottingen, Germany

[2] Univ Gottingen, Abt Allgemeine Mikrobiol, D-37077 Gottingen, Germany

[3] Univ Gottingen, Gottingen Genom Lab, D-37077 Gottingen, Germany

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2000年 / 66卷 / 07期

关键词：

D O I：

10.1128/AEM.66.7.3113-3116.2000

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Environmental DNA libraries prepared from three different soil samples were screened for genes conferring lipolytic activity on Escherichia coli clones. Screening on triolein agar revealed 1 positive clone out of 730,000 clones, and screening on tributyrin agar revealed 3 positive clones out of 286,000 E. coli clones. Substrate specificity analysis revealed that one recombinant strain harbored a lipase and the other three contained esterases. The genes responsible for the lipolytic activity were identified and characterized.

引用

页码：3113 / 3116

页数：4

共 26 条

[1] ESTERASE FROM THE OIL-DEGRADING ACINETOBACTER-LWOFFII RAG-1 - SEQUENCE-ANALYSIS AND OVER-EXPRESSION IN ESCHERICHIA-COLI [J].