Highly sensitive method for amplification of human immunodeficiency virus type 2 DNA

被引：25

作者：

Damond, F

Loussert-Ajaka, I

Apetrei, C

Descamps, D

Souquière, S

Leprêtre, A

Matheron, S

Brun-Vézinet, F

Simon, F

机构：

[1] Hop Bichat Claude Bernard, Virol Lab, F-75018 Paris, France

[2] Hop Bichat Claude Bernard, Serv Malad Infect & Trop, F-75018 Paris, France

[3] Gr T Popa Univ, Sch Med, Dept Microbiol, Virus Lab, Iasi 6600, Romania

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1998年 / 36卷 / 03期

关键词：

D O I：

10.1128/JCM.36.3.809-811.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We evaluated a new human immunodeficiency virus type 2 (HIV-2) DNA amplification strategy based on peripheral blood mononuclear cell long PCR (XL PCR) followed by nested PCR amplification. The primers used were located in the highly conserved long terminal repeat and in the pol regions of the genome. Five primer pairs corresponding to different regions of the HIV-2 env gene were used in the nested step. Samples from 42 patients were tested, which yielded positive amplification with at least two primer pairs in 40 (95%) samples. A primer pair (EB2/EB5) located on the V3 region succeeded in amplifying proviral DNA in 40 samples.

引用

页码：809 / 811

页数：3

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