Intracellular pH sensors based on surface-enhanced Raman scattering

被引：293

作者：

Talley, CE

Jusinski, L

Hollars, CW

Lane, SM

Huser, T

机构：

[1] Lawrence Livermore Natl Lab, Chem & Mat Sci Directorate, Livermore, CA 94550 USA

[2] Lawrence Livermore Natl Lab, Phys & Adv Technol Directorate, Livermore, CA 94550 USA

[3] Univ Calif Davis, NSF, Ctr Biophoton Sci & Technol, Sacramento, CA 95817 USA

[4] Sandia Natl Labs, Livermore, CA 94550 USA

来源：

ANALYTICAL CHEMISTRY | 2004年 / 76卷 / 23期

关键词：

D O I：

10.1021/ac049093j

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We present the development of nanoscale pH sensors based on functionalized silver nanoparticles and surface-enhanced Raman scattering (SERS). The SERS spectrum from individual silver nanoparticle (50-80 nm in diameter) clusters functionalized with 4-mercaptobenzoic acid shows a characteristic response to the pH of the surrounding solution and is sensitive to pH changes in the range of 6-8. Measurements from nanoparticles incorporated in living Chinese hamster ovary cells demonstrate that the nanoparticle sensors retain their robust signal and sensitivity to pH when incorporated into a cell.

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页码：7064 / 7068

页数：5

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