Ensemble and single particle photophysical proper-ties (Two-Photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells

被引:101
作者
Grecco, HE
Lidke, KA
Heintzmann, R
Lidke, DS
Spagnuolo, C
Martinez, OE
Jares-Erijman, EA
Jovin, TM
机构
[1] Univ Buenos Aires, Dept Phys, FCEN, LEC, Buenos Aires, DF, Argentina
[2] Max Planck Inst Biophys Chem, Dept Biol Mol, D-37070 Gottingen, Germany
[3] Univ Buenos Aires, Dept Quim Organ, FCEN, Buenos Aires, DF, Argentina
关键词
nanoparticles; fluorescence microscopy; growth factor receptors;
D O I
10.1002/jemt.20129
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
In this work, we characterized streptavidin-conjugated quantum dots (QDs) manufactured by Quantum Dot Corporation. We present data on: (1) two-photon excitation; (2) fluorescence lifetimes; (3) ensemble and single QD emission anisotropy; (4) QDs as donors for Forster resonance energy transfer (FRET); and (5) spectral conversion of QDs exposed to high-intensity illumination. We also demonstrate the utility of QDs for (1) imaging the binding and uptake of biotinylated transferrin on living cells, and (2) resolving by fluorescence lifetime imaging microscopy (FLIM) signals originating from QDs from those of spatially and spectrally overlapping visible fluorescent proteins (VFPs). (C) 2005 Wiley-Liss. Inc.
引用
收藏
页码:169 / 179
页数:11
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