In Vivo Double and Triple Labeling of Proteins Using Synthetic Amino Acids

被引：55

作者：

Lepthien, Sandra ^{[1
]}

Merkel, Lars ^{[1
]}

Budisa, Nediljko ^{[1
]}

机构：

[1] Max Planck Inst Biochem, D-82152 Martinsried, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 32期

关键词：

amino acid analogues; genetic code; methionine; proline; tryptophan; EXPANDED GENETIC-CODE; ESCHERICHIA-COLI; DESIGN; REASSIGNMENT; REPERTOIRE; METHIONINE; NONSENSE; BARSTAR; BIOLOGY;

D O I：

10.1002/anie.201000439

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Three in one: A new method has been developed for the simultaneous in vivo incorporation of two or three different synthetic amino acids in a protein in a single expression experiment using polyauxotrophic Escherichia coli strains. The picture shows the model protein Ψ-b* used and the synthetic amino acids incorporated. (Figure Presented) © 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：5446 / 5450

页数：5

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