Characterization and diversity of cyanobacterial hepatotoxins (microcystins) in blooms from polish freshwaters identified by liquid chromatography-electrospray ionisation mass spectrometry

被引：49

作者：

Jurczak, T

Tarczyñska, M

Karlsson, K

Meriluoto, J

机构：

[1] Polish Acad Sci, Int Ctr Ecol, PL-05092 Lomianki, Poland

[2] Univ Lodz, Dept Appl Ecol, PL-90237 Lodz, Poland

[3] Abo Akad Univ, Dept Biochem & Pharm, Turku 20521, Finland

来源：

CHROMATOGRAPHIA | 2004年 / 59卷 / 9-10期

关键词：

column liquid chromatography; liquid chromatography-electrospray ionisation mass spectrometry; cyanobacteria; microcystins; Microcystis;

D O I：

10.1365/s10337-004-0279-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Microcystins were determined in 36 cyanobacterial (blue-green algal) bloom samples dominated by Microcystis aeruginosa, Aphanizomenon flos-aquae and Nostoc spp. The analysed samples were collected from 8 water bodies in Poland during summers of 1996-2001. Ten microcystins (MC), three major and seven minor variants, were detected in natural cyanobacterial samples by high-performance liquid chromatography (HPLC). The structures of the microcystins were assigned on the basis of liquid chromatography-electrospray ionisation mass spectrometry (LC-ESI-MS). The main microcystins detected in the blooms from Polish freshwaters were MC-LR, MC-RR and MC-YR. Microcystin content of the blooms varied from a few mug g(-1) to 1687 mug g(-1) freeze-dried material. Also some monodesmethyl (dm) and didesmethyl (dmdm) variants of MC-LR, MC-RR, MC-YR were found in a few analysed samples. Furthermore, two compounds were tentatively characterised as MC-(H-4)YR and dm-MC-FR.

引用

页码：571 / 578

页数：8

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