Ozone induction of ethylene emission in tomato plants:: regulation by differential accumulation of transcripts for the biosynthetic enzymes

被引:104
作者
Tuomainen, J
Betz, C
Kangasjärvi, J
Ernst, D
Yin, ZH
Langebartels, C [1 ]
Sandermann, H
机构
[1] GSF, Natl Res Ctr Environm & Hlth, Inst Biochem Plant Pathol, D-85764 Oberschleissheim, Germany
[2] Univ Kuopio, Dept Ecol & Environm Sci, FIN-70211 Kuopio, Finland
[3] Univ Helsinki, Dept Biosci, Div Genet, FIN-00014 Helsinki, Finland
[4] Univ Helsinki, Inst Biotechnol, FIN-00014 Helsinki, Finland
关键词
D O I
10.1046/j.1365-313X.1997.12051151.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Stress ethylene emission is positively correlated with ozone sensitivity in various plant species, indicating that ethylene may be involved in the control of ozone damage. This study shows that ozone exposure of tomato plants for 5 h at 85 nl l(-1) and above leads to leaf injury within 24 h. 1-aminocyclopropane-1-carboxylic acid (ACC) content and ACC synthase activity were accordingly elevated within 1-2 h. Pre-treatment of leaves with inhibitors of ACC synthase and ACC oxidase significantly inhibited the evolution of ethylene and reduced ozone-induced visible damage. Transcript levels for only one out of three S-adenosyl-L-methionine (SAM) synthetase genes (SAM3), and one out of four ACC synthase genes (LE-ACS2) were induced by ozone (maximum at 2 h). Treatment with protein kinase (K-252a) and phosphatase inhibitors (calyculin A) revealed that ACC synthase activity was additionally regulated by protein phosphorylation/dephosphorylation. Transcripts of ACC oxidase (pTOM13 cDNA probe) displayed the fastest response of the parameters tested (maximum at 30 min), suggesting a regulatory role for ACC oxidase in ethylene formation of ozone-exposed plants. The results demonstrate a highly selective ozone response by ethylene biosynthetic genes which resembles that of plant-pathogen interactions.
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页码:1151 / 1162
页数:12
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