Characterization of NIP2/centrobin, a novel substrate of Nek2, and its potential role in microtubule stabilization

被引:76
作者
Jeong, Yeontae
Lee, Jungmin
Kim, Kyeongmi
Yoo, Jae Cheal
Rhee, Kunsoo [1 ]
机构
[1] Seoul Natl Univ, Dept Biol Sci, Seoul 151742, South Korea
[2] Seoul Natl Univ, Res Ctr Funct Cellul, Seoul 151742, South Korea
关键词
NIP2; centrobin; Nek2; centrosome; mitosis; microtubule;
D O I
10.1242/jcs.03458
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Nek2 is a mitotic kinase whose activity varies during the cell cycle. It is well known that Nek2 is involved in centrosome splitting, and a number of studies have indicated that Nek2 is crucial for maintaining the integrity of centrosomal structure and microtubule nucleation activity. In the present study, we report that NIP2, previously identified as centrobin, is a novel substrate of Nek2. NIP2 was daughter-centriole-specific, but was also found in association with a stable microtubule network of cytoplasm. Ectopic NIP2 formed aggregates but was dissolved by Nek2 into small pieces and eventually associated with microtubules. Knockdown of NIP2 showed significant reduction of microtubule organizing activity, cell shrinkage, defects in spindle assembly and abnormal nuclear morphology. Based on our results, we propose that NIP2 has a role in stabilizing the microtubule structure. Phosphorylation may be crucial for mobilization of the protein to a new microtubule and stabilizing it.
引用
收藏
页码:2106 / 2116
页数:11
相关论文
共 43 条
[1]   Proteomic characterization of the human centrosome by protein correlation profiling [J].
Andersen, JS ;
Wilkinson, CJ ;
Mayor, T ;
Mortensen, P ;
Nigg, EA ;
Mann, M .
NATURE, 2003, 426 (6966) :570-574
[2]   Rootletin forms centriole-associated filaments and functions in centrosome cohesion [J].
Bahe, S ;
Stierhof, YD ;
Wilkinson, CJ ;
Leiss, F ;
Nigg, EA .
JOURNAL OF CELL BIOLOGY, 2005, 171 (01) :27-33
[3]   Polo-like kinase 1 regulates Nlp, a centrosome protein involved in microtubule nucleation [J].
Casenghi, M ;
Meraldi, P ;
Weinhart, U ;
Duncan, PI ;
Körner, R ;
Nigg, EA .
DEVELOPMENTAL CELL, 2003, 5 (01) :113-125
[4]   The dual-specificity phosphatase CDC14B bundles and stabilizes microtubules [J].
Cho, HP ;
Liu, Y ;
Gomez, M ;
Dunlap, J ;
Tyers, M ;
Wang, YS .
MOLECULAR AND CELLULAR BIOLOGY, 2005, 25 (11) :4541-4551
[5]   Assembly of centrosomal proteins and microtubule organization depends on PCM-1 [J].
Dammermann, A ;
Merdes, A .
JOURNAL OF CELL BIOLOGY, 2002, 159 (02) :255-266
[6]   The minus end in sight [J].
Dammermann, A ;
Desai, A ;
Oegema, K .
CURRENT BIOLOGY, 2003, 13 (15) :R614-R624
[7]   Microtubule nucleation and anchoring at the centrosome are independent processes linked by ninein function [J].
Delgehyr, N ;
Sillibourne, J ;
Bornens, M .
JOURNAL OF CELL SCIENCE, 2005, 118 (08) :1565-1575
[8]   Nek2A kinase stimulates centrosome disjunction and is required for formation of bipolar mitotic spindles [J].
Faragher, AJ ;
Fry, AM .
MOLECULAR BIOLOGY OF THE CELL, 2003, 14 (07) :2876-2889
[9]   The Nek2 protein kinase: a novel regulator of centrosome structure [J].
Fry, AM .
ONCOGENE, 2002, 21 (40) :6184-6194
[10]   C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2 [J].
Fry, AM ;
Mayor, T ;
Meraldi, P ;
Stierhof, YD ;
Tanaka, K ;
Nigg, EA .
JOURNAL OF CELL BIOLOGY, 1998, 141 (07) :1563-1574