Phenol metabolism by two microorganisms isolated from Amazonian forest soil samples

被引:27
作者
Bastos, AER [1 ]
Tomisielo, VL
Nozawa, SR
Trevors, JT
Rossi, A
机构
[1] Univ Guelph, Dept Environm Biol, Guelph, ON N1G 2W1, Canada
[2] USP, Fac Filosof Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040901 Ribeirao Preto, SP, Brazil
[3] Univ Sao Paulo, Ctr Energia Nucl Agr, BR-13400970 Piracicaba, SP, Brazil
关键词
phenol; soil; enzyme; extracellular;
D O I
10.1038/sj.jim.7000006
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Two microorganisms isolated from Amazonian forest soil samples and identified as Candida tropicalis and Alcaligenes faecalis were capable of degrading phenol (16 and 12 mM, respectively) at high salt concentrations (15% and 5.6%, respectively). Chromatographic and enzymatic studies revealed that each microorganism cleaved phenol at the ortho position with total phenol mineralization. C-14-phenol mineralization assays showed that both microorganisms assimilated about 30% of the total label. No phenol degradation metabolite (i.e., catechol, cis, cis-muconic acid) was detected in the intercellular medium. The presence of phenol hydroxylase (EC 1.14.13.7) and catechol 1,2-dioxygenase (EC 1.13.11.1) extracellular activity suggested that these microorganisms may secrete these enzymes into the extracellular medium.
引用
收藏
页码:403 / 409
页数:7
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