Development of a Sensitive and Specific Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Blood Thyroglobulin Messenger Ribonucleic Acid in the Follow-Up of Patients with Differentiated Thyroid Carcinoma

被引：18

作者：

Boldarine, Valter T. ^{[1
]}

Maciel, Rui M. B. ^{[1
,2
,3
]}

Guimaraes, Gustavo S. ^{[1
,3
]}

Nakabashi, Claudia C. D. ^{[1
,2
]}

Camacho, Cleber P. ^{[1
,2
]}

Andreoni, Danielle M. ^{[1
,2
]}

Mamone, Maria da Conceicao O. C. ^{[1
,2
]}

Ikejiri, Elza S. ^{[1
,2
]}

Kasamatsu, Teresa S. ^{[1
]}

Crispim, Felipe ^{[1
]}

Hojaij, Flavio C. ^{[1
,2
]}

Hidal, Jairo T. ^{[1
,2
]}

Biscolla, Rosa P. M. ^{[1
,2
,3
]}

机构：

[1] Univ Fed Sao Paulo, Dept Med, Escola Paulista Med, Div Endocrinol,Lab Mol Endocrinol, BR-04039032 Sao Paulo, Brazil

[2] Inst Israelita Ensino & Pesquisa Albert Einstein, Thyroid Dis Ctr, BR-05652000 Sao Paulo, Brazil

[3] Fleury Med & Hlth, BR-01243001 Sao Paulo, Brazil

来源：

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM | 2010年 / 95卷 / 04期

基金：

巴西圣保罗研究基金会;

关键词：

PERIPHERAL-BLOOD; RNA TRANSCRIPTS; MOLECULAR-DETECTION; CANCER PATIENTS; REAL-TIME; RT-PCR; CELLS; MANAGEMENT; DISEASE; RECURRENCES;

D O I：

10.1210/jc.2009-1354

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Context: Serum thyroglobulin is a sensitive tumor marker in the follow-up of patients with differentiated thyroid carcinoma (DTC), but the presence of endogenous anti-thyroglobulin antibodies (TgAb) can interfere on its measurement. To prevent interference by TgAb, several investigators have tried to quantify blood mRNA Tg by real-time RT-PCR, but the results have been variable, not reporting a correlation between mRNA Tg and the presence of metastases. Objective: The aim of the study was to evaluate the development of a sensitive and specific quantitative RT-PCR assay for blood mRNA Tg in the follow-up of patients with DTC. Design and Patients: An assay employing primers located in a region not affected by alternative splicing or single nucleotide polymorphisms was developed to study 104 DTC patients (13 of 104 with positive TgAb). Results: The assay is specific for thyroid tissue because we found mRNA Tg expression in normal thyroid tissue, but we did not find any mRNA Tg expression in any extrathyroidal tissues. Quantitative mRNA Tg levels were significantly different between patients "free of disease" (82 of 104) and those with metastases (22 of 104) (2.61 +/- 0.26 vs. 27.58 +/- 1.62 pg mRNA Tg/mu g RNA) (P < 0.0001). A cutoff point of 5.51 was able to discriminate between the two groups. In addition, the measurement of mRNA Tg was not affected by the presence of TgAb. Conclusion: This new mRNA Tg quantification is a reliable method that allowed us to differentiate patients free of disease from those with metastases, and it could represent an appropriate molecular marker for the follow-up of patients with DTC, especially those with positive TgAb. (J Clin Endocrinol Metab 95: 1726-1733, 2010)

引用

页码：1726 / 1733

页数：8

共 37 条

[1]

[Anonymous], 2003, THYROID, V13, P3

[2]

Bellantone R, 2001, CANCER-AM CANCER SOC, V92, P2273, DOI 10.1002/1097-0142(20011101)92:9<2273::AID-CNCR1573>3.0.CO

[3]

2-Y

[4] DEMONSTRATION OF A HETEROGENEOUS TRANSCRIPTION PATTERN OF THYROGLOBULIN MESSENGER-RNA IN HUMAN THYROID TISSUES [J].