Anti-mesothelial markers in sarcomatoid mesothelioma and other spindle cell neoplasms

被引:90
作者
Attanoos, RL
Dojcinov, SD
Webb, R
Gibbs, AR
机构
[1] Llandough Hosp, Dept Histopathol, Cardiff, S Glam, Wales
[2] Univ Hosp Wales NHS Trust, Penarth, S Glam, Wales
关键词
calretinin; cytokeratin; 5/6; mesothelioma; sarcoma; spindle cell neoplasms; thrombomodulin;
D O I
10.1046/j.1365-2559.2000.00981.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Aims: To undertake a comparative evaluation of three antimesothelial markers (thrombomodulin, cytokeratin 5/6 and calretinin) with broad spectrum cytokeratin (AE1/AE3) in differentiating between sarcomatoid mesothelioma and a spectrum of spindle cell neoplasms. Methods and results: Thirty-one malignant sarcomatoid mesotheliomas were studied. Calretinin expression was focally identified in 12 (39%) tumours and thrombomodulin and cytokeratin 5/6 immunoreactivity was seen in nine (29%) cases. In comparison there was strong diffuse cytoplasmic reactivity with the broad spectrum cytokeratin (AE1/AE3) in 24 of 31 (77%) tumours. Thirty mixed spindle cells neoplasms were studied. No calretinin expression was identified in any case. Thrombomodulin immunoreactivity was identified in four (16%) cases (two angiosarcomas, two high-grade sarcomas, not otherwise specified). Cytokeratin 5/6 expression was seen in one high-grade pulmonary sarcoma originally termed malignant fibrous histiocytoma. None of the antimesothelial markers was expressed in the four spindle cell carcinomas studied. In contrast, broad spectrum cytokeratin was diffusely expressed in all four spindle cell carcinomas (three pulmonary, one renal), both synovial sarcomas, both malignant mixed Mullerian tumours, one of three pulmonary leiomyosarcomas and two of nine sarcomas, not otherwise specified. Conclusions: Immunohistochemistry has a more limited role in the diagnosis and distinction of sarcomatoid mesothelioma from other spindle cell neoplasms. The combination of a broad spectrum cytokeratin with calretinin combines both high sensitivity (77% for AE1/AE3) with high specificity (100% for calretinin) for sarcomatoid mesothelioma and can be diagnostically useful. The mesothelial markers, thrombomodulin and cytokeratin 5/6, are not useful alone in the diagnosis of sarcomatoid mesothelioma as each shows insufficient antibody sensitivity, although together they complement calretinin.
引用
收藏
页码:224 / 231
页数:8
相关论文
共 44 条
[1]   PLEURAL MESOTHELIOMA OF CONNECTIVE-TISSUE TYPE, LOCALIZED FIBROUS TUMOR OF THE PLEURA, AND REACTIVE SUBMESOTHELIAL HYPERPLASIA - AN IMMUNOHISTOCHEMICAL COMPARISON [J].
ALIZZI, M ;
THURLOW, NP ;
CORRIN, B .
JOURNAL OF PATHOLOGY, 1989, 158 (01) :41-44
[2]  
ALVAREZFERNANDEZ E, 1979, CANCER, V43, P1658, DOI 10.1002/1097-0142(197905)43:5<1658::AID-CNCR2820430515>3.0.CO
[3]  
2-Y
[4]  
Appleton M. A. C., 1995, Journal of Pathology, V176, p26A
[5]   Primary epithelioid haemangioendothelioma of the peritoneum: An unusual mimic of diffuse malignant mesothelioma [J].
Attanoos, RL ;
Dallimore, NS ;
Gibbs, AR .
HISTOPATHOLOGY, 1997, 30 (04) :375-377
[6]   CD44H expression in reactive mesothelium, pleural mesothelioma and pulmonary adenocarcinoma [J].
Attanoos, RL ;
Webb, R ;
Gibbs, AR .
HISTOPATHOLOGY, 1997, 30 (03) :260-263
[7]   Mesothelioma-binding antibodies: Thrombomodulin, OV 632 and HBME-1 and their use in the diagnosis of malignant mesothelioma [J].
Attanoos, RL ;
Goddard, H ;
Gibbs, AR .
HISTOPATHOLOGY, 1996, 29 (03) :209-215
[8]  
AZUMI N, 1989, LAB INVEST, V60, pA4
[9]  
BATTIFORA H, 1995, ATLAS TUMOUR PATHOL, P37
[10]   CYTOKERATIN EXPRESSION IN SMOOTH-MUSCLE AND SMOOTH-MUSCLE TUMORS [J].
BROWN, DC ;
THEAKER, JM ;
BANKS, PM ;
GATTER, KC ;
MASON, DY .
HISTOPATHOLOGY, 1987, 11 (05) :477-486