Small Silencing RNAs in Plants Are Mobile and Direct Epigenetic Modification in Recipient Cells

被引：538

作者：

Molnar, Attila ^{[1
]}

Melnyk, Charles W. ^{[1
]}

Bassett, Andrew ^{[1
]}

Hardcastle, Thomas J. ^{[1
]}

Dunn, Ruth ^{[1
]}

Baulcombe, David C. ^{[1
]}

机构：

[1] Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England

来源：

SCIENCE | 2010年 / 328卷 / 5980期

基金：

加拿大自然科学与工程研究理事会; 欧洲研究理事会;

关键词：

POTATO-VIRUS-X; DNA-METHYLATION; POLYMERASE-IV; ARABIDOPSIS-THALIANA; INTERFERING RNA; SIGNAL; EXPRESSION; SEQUENCE; SIRNAS; PHLOEM;

D O I：

10.1126/science.1187959

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A silencing signal in plants with an RNA specificity determinant moves through plasmodesmata and the phloem. To identify the mobile RNA, we grafted Arabidopsis thaliana shoots to roots that would be a recipient for the silencing signal. Using mutants that block small RNA (sRNA) biogenesis in either source or recipient tissue, we found that transgene-derived sRNA as well as a substantial proportion of the endogenous sRNA had moved across the graft union, and we provide evidence that 24-nucleotide mobile sRNAs direct epigenetic modifications in the genome of the recipient cells. Mobile sRNA thus represents a mechanism for transmitting the specification of epigenetic modification and could affect genome defense and responses to external stimuli that have persistent effects in plants.

引用

页码：872 / 875

页数：4

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