A third active AKH is present in the pyrgomorphid grasshoppers Phymateus morbillosus and Dictyophorus spumans

被引：20

作者：

Siegert, KJ

Kellner, R

Gäde, G

机构：

[1] Univ Cape Town, Dept Zool, ZA-7701 Rondebosch, South Africa

[2] Merck KGaA, Biomed Fo GBT, D-64271 Darmstadt, Germany

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2000年 / 30卷 / 11期

基金：

英国生物技术与生命科学研究理事会; 新加坡国家研究基金会;

关键词：

adipokinetic; hyperlipaemic; pyrgomorphid; grasshopper; locust;

D O I：

10.1016/S0965-1748(00)00081-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The corpora cardiaca of the African pyrgomorphid grasshoppers Phymateus morbillosus and Dictyophorus spumans contain three adipokinetic hormones (AKHs): besides two already known AKHs, Phm-AKH-I and Scg-AKH-II (Gade et al., 1996 [Gade, G., Kellner, R., Rinehart, K.L., 1996. Pyrgomorphid grasshoppers of the genus Phymateus contain species-specific decapeptides of the AKH/RPCH family regulating lipid-mobilisation during flight. Physiol. Entomol. 21, 193-202]), a new AI(H-LU, denoted Phm-AKH-III, pGlu-Ile-Asn-Phe-Thr-Pro-Trp-Trp-NH2, has been characterised. This is only the second AKH-III identified so far, thus, only three: insect species - all of them grasshoppers - contain three active AKHs. Phm-AKH-III differs from Lom-AKH-III from the migratory locust, Locusta migratoria, only in position 2: isoleucine is present instead of leucine. The structure of the Phm-AKH-III was confirmed by synthesis, subsequent mass determination and reversed-phase high-performance liquid chromatography. The synthetic peptide also induced hyperlipaemia in D. spumans and L. migratoria. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：1061 / 1067

页数：7

共 31 条

[1] MOLECULAR-CLONING OF 3 DISTINCT CDNAS, EACH ENCODING A DIFFERENT ADIPOKINETIC HORMONE PRECURSOR, OF THE MIGRATORY LOCUST, LOCUSTA-MIGRATORIA - DIFFERENTIAL EXPRESSION OF THE DISTINCT ADIPOKINETIC HORMONE PRECURSOR GENES DURING FLIGHT ACTIVITY [J].