Anti-adhesive glycoproteins in echinoderm mucus secretions

被引:45
作者
Bavington, CD
Lever, R
Mulloy, B
Grundy, MM
Page, CP
Richardson, NV
McKenzie, JD
机构
[1] Dunstaffnage Marine Lab, Scottish Assoc Marine Sci, Oban PA34 4AD, Argyll, Scotland
[2] Kings Coll London, Sackler Inst Pulm Pharmacol, London SE1 9RT, England
[3] Natl Inst Biol Stand & Controls, Potters Bar EN6 3QG, Herts, England
[4] Univ St Andrews, Sch Chem, St Andrews KY16 9ST, Fife, Scotland
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2004年 / 139卷 / 04期
基金
英国自然环境研究理事会;
关键词
mucus; glycoprotein; mucin; echinoderm; adhesion; leukocyte; Pseudomonas; biofouling;
D O I
10.1016/j.cbpc.2004.07.008
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Marine invertebrates produce a large variety of mucus secretions which are rich in glycoproteins. As part of our studies of natural antifouling mechanisms, mucus secretions from the starfish Marthasterias glacialis and Porania pulvillus and the brittlestar Ophiocomina nigra have been used to characterise the structure and function of some of the glycoproteins present in these secretions. Mucus was collected from all three species and fractionated by size exclusion chromatography. A high molecular weight glycoprotein fraction was collected from each species. Monosaccharide analysis and FTIR demonstrated a composition consistent with a mucin-type glycoprotein. The mucin from M glacialis and O. nigra inhibited in vitro bacterial adhesion in a dose-dependent manner. In contrast, the mucin from P. pulvillus promoted bacterial adhesion in a dose-dependent manner. All of the mucins inhibited the adhesion of human neutrophils to cultured human vascular endothelial cells (HUVECs) and had no anticoagulant activity. The mucins described here have adhesion-regulating functions that may have a role in the antifouling or feeding mechanisms of the organisms that produce them. These mucins may also be of therapeutic value through their ability to regulate human neutrophil adhesion or bacterial adhesion. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:607 / 617
页数:11
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