Interaction of human Ku70 with TRF2

被引：139

作者：

Song, K

Jung, D

Jung, Y

Lee, SG

Lee, I ^{[1
]}

机构：

[1] Univ Ulsan, Coll Med, Dept Pathol, Seoul 138736, South Korea

[2] Univ Ulsan, Coll Med, Dept Biochem, Seoul 138736, South Korea

[3] Asan Inst Life Sci, Songpa Gu, Seoul 138736, South Korea

来源：

FEBS LETTERS | 2000年 / 481卷 / 01期

关键词：

yeast two-hybrid; Ku; TRF2; telomere;

D O I：

10.1016/S0014-5793(00)01958-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ku, a heterodimer of 70- and 80-kDa subunits, plays a general role in the metabolism of DIVA ends in eukaryotic cells, including double-strand DNA break repair, V(D)J recombination, and maintenance of telomeres, We have utilized the yeast two-hybrid system to identify Ku70-interacting proteins other than Ku80, Two reactive clones were found to encode the dimerization domain of TRF2, a mammalian telomeric protein that binds to duplex TTAGGG repeats at chromosome ends. This interaction was confirmed using bacterial fusion proteins and co-immunoprecipitations from eukaryotic cells overexpressing TRF2, The transfected TFR2 colocalized with Ku70, (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. all rights reserved.

引用

页码：81 / 85

页数：5

共 32 条

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