Green fluorescent protein-tagged retroviral envelope protein for analysis of virus-cell interactions

被引：13

作者：

Spitzer, D

Dittmar, KEJ

Rohde, M

Hauser, H ^{[1
]}

Wirth, D

机构：

[1] German Res Ctr Biotechnol, Dept Gene Regulat & Differentiat, D-38124 Braunschweig, Germany

[2] German Res Ctr Biotechnol, Dept Microbial Pathogenesis & Vaccine Res, D-38124 Braunschweig, Germany

来源：

JOURNAL OF VIROLOGY | 2003年 / 77卷 / 10期

关键词：

D O I：

10.1128/JVI.77.10.6070-6075.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Fluorescent retroviral envelope (Env) proteins were developed for direct visualization of viral particles. By fusing the enhanced green fluorescent protein (eGFP) to the N terminus of the amphotropic 4070A envelope protein, extracellular presentation of eGFP was achieved. Viruses incorporated the modified Env protein and efficiently infected cells. We used the GFP-tagged viruses for staining retrovirus receptor-positive cells, thereby circumventing indirect labeling techniques. By generating cells which conditionally expressed the GFP-tagged Env protein, we could confirm an inverse correlation between retroviral Env expression and infectivity (superinfection). eGFP-tagged virus particles are suitable for monitoring the dynamics of virus-cell interactions.

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收藏

页码：6070 / 6075

页数：6

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