Respiration of arsenate and selenate by hyperthermophilic archaea

被引:152
作者
Huber, R
Sacher, M
Vollmann, A
Huber, H
Rose, D
机构
[1] Univ Regensburg, Lehrstuhl Mikrobiol, D-93053 Regensburg, Germany
[2] Univ Regensburg, Archaeenzentrum, D-93053 Regensburg, Germany
[3] Univ Regensburg, Fak Chem & Pharm, Betriebseinheit Materialuntersuchung, D-8400 Regensburg, Germany
关键词
Pyrobaculum arsenaticum; hyperthermophile; solfataric; biogeochemistry-Realgar; arsenic; arsenate respiration; selenate respiration;
D O I
10.1016/S0723-2020(00)80058-2
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A novel, strictly anaerobic, hyperthermophilic, facultative organotrophic archaeon was isolated from a hut spring at Pisciarelli Solfatara, Naples, Italy. The rod-shaped cells grew chemolithoautotrophically with carbon dioxide as carbon source, hydrogen as electron donor and arsenate, thiosulfate or elemental sulfur as electron acceptor. H2S was formed from sulfur or thiosulfate, arsenite from arsenate. Organotrophically, the new isolate grew optimally in the presence of an inorganic electron acceptor like sulfur, selenate or arsenate. Cultures, grown on arsenate and thiosulfate: or arsenate and L-cysteine, precipitated realgar (As2S2). During growth on selenate, elemental selenium was produced. The G+C content of the DNA was 58.3 mol%. Due to 16S rRNA gene sequence analysis combined with physiological and morphological criteria, the new isolate belongs to the Thermoproteales order. It represents a new species within the genus Pyrobaculum, the type species of which we name Pyrobaculum arsenaticum (type strain PZ6*, DSM 13514, ATCC 700994). Comparative studies with different Pyrobaculum-species showed, that Pyrobaculum aerophilum was also able to grow organotrophically under anaerobic culture conditions in the presence of arsenate, selenate and selenite. During growth on selenite, elemental selenium was formed as final product. In contrast to P, arsenaticum, P. aerophilum could use selenate or arsenate for lithoautotrophic growth with carbon dioxide and hydrogen.
引用
收藏
页码:305 / 314
页数:10
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