Superheated water chromatography-nuclear magnetic resonance spectroscopy and mass spectrometry of vitamins

被引：33

作者：

Chienthavorn, O

Smith, RM ^{[1
]}

Saha, S

Wilson, ID

Wright, B

Taylor, SD

Lenz, EM

机构：

[1] Univ Loughborough, Dept Chem, Loughborough LE11 3TU, Leics, England

[2] AstraZeneca, Macclesfield SK10 4TG, Cheshire, England

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2004年 / 36卷 / 03期

关键词：

vitamin; riboflavin; superheated water; HPLC; LC-NMR;

D O I：

10.1016/j.jpba.2004.07.023

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The water-soluble vitamins, pyridoxine, riboflavin, and thiamine, were separated by reversed-phase liquid chromatography using hot or superheated water as the mobile phase and were detected using a range of detectors, including ultraviolet and fluorescence spectroscopy and mass spectrometry. By using deuterium oxide as the eluent, direct on-line nuclear magnetic resonance spectra could be obtained with minimal spectral interference from the mobile phase. Some of the Compounds showed deuterium exchange of alkyl-protons when separated at high temperatures. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：477 / 482

页数：6

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