Development of a gas-phase biosensor for trimethylamine using a flavin-containing monooxygenase 3

被引：17

作者：

Mitsubayashi, K ^{[1
]}

Hashimoto, Y ^{[1
]}

机构：

[1] Tokai Univ, Sch Engn, Dept Elect Engn, Hiratsuka, Kanagawa 2591292, Japan

来源：

ELECTROCHEMISTRY | 2000年 / 68卷 / 11期

关键词：

gas-phase biosensor; trimethylamine; flavin-containing monooxygenase;

D O I：

10.5796/electrochemistry.68.901

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

Biosensors for trimethylamine (TMA) in the liquid- and gas-phases were constructed using a flavin-containing monooxygenase 3 (FMO3) and a substrate regeneration cycle with reducing agents. The enzyme electrode for TMA in the liquid-phase was calibrated against TMA solutions from 0.5 to 175 mmol.l(-1), covering the concentration range encountered in the corrupt fish-flesh. The gas-phase biosensor for TMA, fabricated by incorporating the enzyme electrode into a reaction cell with both gas- and liquid-phase compartments separated a porous PTFE diaphragm membrane, could be used to measure TMA in the gas phase with high selectivity.

引用

页码：901 / 903

页数：3

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