Murine gammaherpesvirus 68 contains two functional lytic origins of replication

被引：8

作者：

Adler, Heiko

Steer, Beatrix

Freimueller, Klaus

Haas, Juergen

机构：

[1] GSF Munich, Natl Res Ctr Environm & Hlth, Clin Cooperat Grp Hematopoiet Cell Transplantat, Inst Mol Immunol, D-81377 Munich, Germany

[2] Univ Munich, Dept Med 3, Munich, Germany

[3] Univ Munich, Max Von Pettenkofer Inst, Munich, Germany

来源：

JOURNAL OF VIROLOGY | 2007年 / 81卷 / 13期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1128/JVI.02406-06

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A 1.25-kbp DNA fragment from the right side of the genome containing the lytic origin of replication (oriLyt) of murine gammaherpesvirus 68 (MHV-68) has been identified by a plasmid replication assay. Here we show that a mutant MHV-68 with a deletion of an essential part of this oriLyt, generated by using an MHV-68 bacterial artificial chromosome, was only slightly attenuated and still able to replicate but that a mutant containing an additional deletion on the left side of the genome was replication deficient. The newly identified region was sufficient to support plasmid replication, thus providing evidence for a second oriLyt.

引用

页码：7300 / 7305

页数：6

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