Expression patterns of laminin α1 and α5 in human lung during development

被引:33
作者
Pierce, RA
Griffin, GL
Miner, JH
Senior, RM
机构
[1] Barnes Jewish Hosp, Dept Med, Div Pulm & Crit Care Med, St Louis, MO 63110 USA
[2] Barnes Jewish Hosp, Dept Med, Div Renal Dis, St Louis, MO 63110 USA
[3] Barnes Jewish Hosp, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
[4] Washington Univ, Sch Med, St Louis, MO USA
关键词
D O I
10.1165/ajrcmb.23.6.4202
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Laminins are trimeric glycoprotein components of basement membranes. Each laminin has three structurally similar chains, designated alpha, beta, and gamma. Five laminin alpha chains are now known. In previous studies using monoclonal antibody 4C7, laminin al was thought to be present in basement membranes of human lung throughout development and in the adult, but recent expression studies have demonstrated that 4C7 identifies laminin alpha5 rather than alpha1. To determine the temporal and spatial patterns of laminin alpha1 and laminin alpha5 in developing human lung, we prepared complementary DNA probes specific for laminin alpha1 and alpha5 messenger RNAs (mRNAs). By Northern analysis, laminin alpha1 mRNA was prominent in first-trimester fetal lung, but was not detectable at 23 wk or at later times. In contrast, laminin alpha5 mRNA was readily detected in early fetal lung and remained present thereafter. Immunohistochemical staining demonstrated laminin alpha1 only in early fetal lung, whereas laminin alpha5 was persistent from the early fetal period. In situ hybridization localized laminin al expression to distal epithelium in the first-trimester lung, and laminin alpha5 to all epithelium and developing pulmonary arteries from the first trimester through the perinatal period. These studies indicate that laminin al expression is restricted to early human lung morphogenesis, whereas the expression of laminin alpha5 in human lung is continuous from early lung development through adult life. It is evident that laminin alpha1 and laminin alpha5 have different roles in the development of the human lung.
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页码:742 / 747
页数:6
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