hupS and hupL constitute a transcription unit in the cyanobacterium Nostoc sp PCC 73102

被引：23

作者：

Lindberg, P

Hansel, A

Lindblad, P

机构：

[1] Uppsala Univ, EBC, Dept Physiol Bot, S-75236 Uppsala, Sweden

[2] Univ Jena, Fac Med, Mol & Cellular Biophys Res Unit, D-07747 Jena, Germany

来源：

ARCHIVES OF MICROBIOLOGY | 2000年 / 174卷 / 1-2期

关键词：

cyanobacteria; Nostoc; uptake hydrogenase; hupSL; transcription; 5 '-RACE; RT-PCR; STRR;

D O I：

10.1007/s002030000186

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The heterocystous cyanobacterium Nostoc sp. PCC 73102 possesses an uptake hydrogenase encoded by hupS and hupL.. The genes are transcribed in cells grown under N-2-fixing but not under non-N-2-fixing conditions. We characterised the transcription of hupS and hupL in further detail. A transcription start site was located at a position 259 bp upstream from the hupS translation start. As shown by RT-PCR, hnpS and hupL constitute a single transcript in Nostoc sp. PCC 73102. The possible function of short, tandemly repeated repetitive sequences between hupS and hupL, enabling a folding of the entire intergenic region into a hairpin, is discussed.

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页码：129 / 133

页数：5

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