Mosquito dopa decarboxylase cDNA characterization and blood-meal-induced ovarian expression

被引:35
作者
Ferdig, MT [1 ]
Li, J [1 ]
Severson, DW [1 ]
Christensen, BM [1 ]
机构
[1] UNIV WISCONSIN,DEPT ANIM HLTH & BIOMED SCI,MADISON,WI
关键词
Aedes aegypti; dopa decarboxylase; gene expression; ovary development;
D O I
10.1111/j.1365-2583.1996.tb00046.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Dopa decarboxylase (DDC) functions in insect catecholamine biochemistry to produce materials essential for cross-linking reactions that result in tanning and/or melanization, including tanning of the mosquito egg chorion and encapsulation of parasites. We have cloned Dde from the mosquito, Aedes aegypti, and studied its expression in response to blood-feeding, which initiates events necessary for egg maturation in mosquitoes. The Ae. aegypti Dde cDNA was isolated via heterologous screening using a clone from Drosophila melanogaster. A resulting 1.87 kilobase (kb) clone was sequenced to reveal an open reading frame of 1464 bp, as well as 5'- and 3'-untranslated segments, The inferred amino acid sequence of this clone shares 81% identity with the published Drosophila Dde cDNA, including complete identity with twenty-four contiguous amino acids encompassing the pyridoxal-5-phosphate cofactor binding domain, Analysis of an F2 intercross population derived from a parental cross between two Ae. aegypti strains (Hamburg and Moyo-In-Dry) allowed us to map Dde to a locus on linkage group 2. Expression studies demonstrated the presence of a 2.1 kb message, the majority of which occurs in the ovaries where Ddc-specific mRNA is up-regulated in response to ingestion of a blood meal, The potential for egg-tanning in anautogenous mosquitoes as a model for understanding specific genetic events in the regulation of catecholamine metabolism is addressed.
引用
收藏
页码:119 / 126
页数:8
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