Effects of medium glucose concentration and pH on docosahexaenoic acid content of heterotrophic Crypthecodinium cohnii

被引：63

作者：

Jiang, Y ^{[1
]}

Chen, F ^{[1
]}

机构：

[1] Univ Hong Kong, Dept Bot, Hong Kong, Hong Kong, Peoples R China

来源：

PROCESS BIOCHEMISTRY | 2000年 / 35卷 / 10期

关键词：

Crypthecodinium cohnii; heterotrophic growth; docosahexaenoic acid; fatty acid composition; pH; glucose;

D O I：

10.1016/S0032-9592(00)00163-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of medium glucose concentration and pH on growth and docosahexaenoic acid (DHA, C22:6 omega-3) content of Crypthecodinium cohnii were investigated. Over a range of glucose concentrations (5-40 g l(-1)) investigated, the highest specific growth rate (0.12 h(-1)), highest cell dry weight concentration (3.13 g l(-1)) and highest growth yield on glucose (0.6 g g(-1)) were obtained at 20 g l(-1) glucose. However, the highest degree of fatty acid unsaturation (3.2) and highest DHA proportion (53.4% of total fatty acids) were achieved at 5 g l(-1) glucose. Low glucose concentrations enhanced the degree of fatty acid unsaturation and DHA formation. Medium pH also affected cell growth, fatty acid unsaturation and DHA proportion. When medium pH was 7.2, the highest specific growth rate (0.089 h(-1)), highest cell dry weight concentration (2.73 g l(-1)), highest growth yield on glucose (0.564 g g(-1)), highest degree of Fatty acid unsaturation (3.4) and highest DHA proportion (56.8% of total fatty acids) were obtained. Results suggest that glucose concentration and pH value could be effectively manipulated to achieve maximum DHA production by C. cohnii. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：1205 / 1209

页数：5

共 22 条

[1] ENVIRONMENTAL INFLUENCES ON DOCOSAHEXAENOATE CONTENT OF TRIACYLGLYCEROLS AND PHOSPHATIDYLCHOLINE OF A HETEROTROPHIC, MARINE DINOFLAGELLATE, CRYPTHECODINIUM-COHNII [J].

BEACH, DH ;

HOLZ, GG .

BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 316 (01) :56-65

[2]

BEACH DH, 1973, THESIS STATE U NEW Y

[3] MOLECULAR-SPECIES COMPOSITION OF PHOSPHATIDYLCHOLINE FROM CRYPTHECODINIUM-COHNII IN RELATION TO GROWTH TEMPERATURE [J].

BELL, MV ;

HENDERSON, RJ .

LIPIDS, 1990, 25 (02) :115-118

[4] Preclinical evaluation of single-cell oils that are highly enriched with arachidonic acid and docosahexaenoic acid [J].

Boswell, K ;

Koskelo, EK ;

Carl, L ;

Glaza, S ;

Hensen, DJ ;

Williams, KD ;

Kyle, DJ .

FOOD AND CHEMICAL TOXICOLOGY, 1996, 34 (07) :585-593

[5] Relationship between substrate inhibition and maintenance energy of Chlamydomonas reinhardtii in heterotrophic culture [J].

Chen, F ;

Johns, MR .

JOURNAL OF APPLIED PHYCOLOGY, 1996, 8 (01) :15-19

[6]

CHEN F, 1991, Journal of Applied Phycology, V3, P203, DOI 10.1007/BF00003578

[7] Fatty acid unsaturation in the red alga Porphyridium cruentum. Is the methylene interrupted nature of polyunsaturated fatty acids an intrinsic property of the desaturases? [J].

Cohen, Z ;

Shiran, D ;

Khozin, I ;

Heimer, YM .

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1997, 1344 (01) :59-64

[8] Optimisation of docosahexaenoic acid production in batch cultivations by Crypthecodinium cohnii [J].

de Swaaf, ME ;

de Rijk, TC ;

Eggink, G ;

Sijtsma, L .

JOURNAL OF BIOTECHNOLOGY, 1999, 70 (1-3) :185-192

[9] Polyunsaturated fatty acids .1. Occurrence, biological activities and applications [J].

Gill, I ;

Valivety, R .

TRENDS IN BIOTECHNOLOGY, 1997, 15 (10) :401-409

[10] LIPID-COMPOSITION AND BIOSYNTHESIS IN THE MARINE DINOFLAGELLATE CRYPTHECODINIUM-COHNII [J].

HENDERSON, RJ ;

LEFTLEY, JW ;

SARGENT, JR .

PHYTOCHEMISTRY, 1988, 27 (06) :1679-1683

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