FLOWERING LOCUS T protein may act as the long-distance florigenic signal in the cucurbits

被引:369
作者
Lin, Ming-Kuem
Belanger, Helene
Lee, Young-Jin
Varkonyi-Gasic, Erika
Taoka, Ken-Ichiro
Miura, Eriko
Xoconostle-Cazares, Beatriz
Gendler, Karla
Jorgensene, Richard A.
Phinney, Brett
Lough, Tony J.
Lucas, William J. [1 ]
机构
[1] Univ Calif Davis, Coll Biol Sci, Plant Biol Sect, Davis, CA 95616 USA
[2] Agrigenesis Biosci, Auckland 1140, New Zealand
[3] Univ Calif Davis, Genome Ctr, Proteom Core Facil, Davis, CA 95616 USA
[4] Ctr Invest & Avanzados Inst Politecn Nacl, Dept Biotechnol & Bioingn, San Pedro Zacatenco, Mexico
[5] Univ Arizona, Dept Plant Sci, Tucson, AZ 85721 USA
关键词
D O I
10.1105/tpc.107.051920
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cucurbita moschata, a cucurbit species responsive to inductive short-day (SD) photoperiods, and Zucchini yellow mosaic virus (ZYMV) were used to test whether long-distance movement of FLOWERING LOCUS T (FT) mRNA or FT is required for floral induction. Ectopic expression of FT by ZYMV was highly effective in mediating floral induction of long-day (LD)-treated plants. Moreover, the infection zone of ZYMV was far removed from floral meristems, suggesting that FT transcripts do not function as the florigenic signal in this system. Heterografting demonstrated efficient transmission of a florigenic signal from flowering Cucurbita maxima stocks to LD-grown C. moschata scions. Real-time RT-PCR performed on phloem sap collected from C. maxima stocks detected no FT transcripts, whereas mass spectrometry of phloem sap proteins revealed the presence of Cm-FTL1 and Cm-FTL2. Importantly, studies on LD- and SD-treated C. moschata plants established that Cmo-FTL1 and Cmo-FTL2 are regulated by photoperiod at the level of movement into the phloem and not by transcription. Finally, mass spectrometry of florally induced heterografted C. moschata scions revealed that C. maxima FT, but not FT mRNA, crossed the graft union in the phloem translocation stream. Collectively, these studies are consistent with FT functioning as a component of the florigenic signaling system in the cucurbits.
引用
收藏
页码:1488 / 1506
页数:19
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