Multiplex nested reverse transcription-polymerase chain reaction for respiratory viruses in acute otitis media

被引：7

作者：

Ishibashi, T

Monobe, H

Nomura, Y

Shinogami, M

Yano, J

机构：

[1] Social Insurance Cent Gen Hosp, Dept Otolaryngol, Shinjyuku Ku, Tokyo 1690073, Japan

[2] Japanese Red Cross, Med Ctr, Dept Otolaryngol, Tokyo, Japan

[3] Univ Tokyo, Dept Otolaryngol, Tokyo 113, Japan

来源：

ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY | 2003年 / 112卷 / 03期

关键词：

acute otitis media; clinical laboratory diagnosis; respiratory virus; reverse transcription-polymerase chain reaction;

D O I：

10.1177/000348940311200311

中图分类号：

R76 [耳鼻咽喉科学];

学科分类号：

100213 ;

摘要：

Because respiratory viruses play an important role in the causation and pathogenesis of acute otitis media (AOM), determining which virus has infected a child is important with respect to vaccines and antiviral drugs. In some instances, this information might be used to prevent the occurrence of AOM. We used a rapid, economical, and sensitive diagnostic system involving a multiplex nested reverse transcription-polymerase chain reaction (RT-PCR) assay to detect various respiratory viruses in clinical specimens of middle ear fluid (MEF) from children with AOM in our hospital. Multiplex RT-PCR was completed on 40 MEF samples from 28 infants and children less than 6 years old with AOM. Viral RNA was detected in 17 MEF samples (43%). Respiratory syncytial virus type A was present in 12 samples, adenovirus in 3, rhinovirus in 2, and influenza A (H3N2) in 1. The multiplex RT-PCR assay is recommended to clinical laboratories that are considering adoption of a molecular technique for viral diagnosis.

引用

页码：252 / 257

页数：6

共 15 条

[1] RESPIRATORY VIRUS-INFECTION AS A CAUSE OF PROLONGED SYMPTOMS IN ACUTE OTITIS-MEDIA [J].