Identification of a putative receptor for porcine reproductive and respiratory syndrome virus on porcine alveolar macrophages

被引：86

作者：

Duan, XB ^{[1
]}

Nauwynck, HJ ^{[1
]}

Favoreel, HW ^{[1
]}

Pensaert, MB ^{[1
]}

机构：

[1] State Univ Ghent, Fac Vet Med, Lab Vet Virol, B-9820 Merelbeke, Belgium

来源：

JOURNAL OF VIROLOGY | 1998年 / 72卷 / 05期

关键词：

D O I：

10.1128/JVI.72.5.4520-4523.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To identify the receptor which may determine the macrophage tropism of porcine reproductive and respiratory syndrome virus (PRRSV), monoclonal antibodies (MAbs) against porcine alveolar macrophages (PAM) were produced. Two MAbs (41D3 and 41D5) which completely blocked PRRSV infection of PAM were further characterized. It was found that they reduce the attachment of PRRSV to PAM and immunoprecipitate a 210-kDa membrane protein from PAM. This protein was detected on the cell membranes of PAM but not of PRRSV-nonpermissive cells. A colocalization was found between the reactive sites of MAb 41D3 and PRRSV on PAM membranes. All PRRSV-infected cells in tissues of experimentally infected pigs reacted with MAb 41D3. Taken together, all these data suggest that the identified 210-kDa membrane protein is a putative receptor for PRRSV on porcine macrophages.

引用

页码：4520 / 4523

页数：4

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