A putative spectrin-containing membrane skeleton in hyphal tips of Neurospora crassa

被引：22

作者：

Degousée, N ^{[1
]}

Gupta, GD ^{[1
]}

Lew, RR ^{[1
]}

Heath, IB ^{[1
]}

机构：

[1] York Univ, Dept Biol, Toronto, ON M3J 1P3, Canada

来源：

FUNGAL GENETICS AND BIOLOGY | 2000年 / 30卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

tip growth; spectrin; membrane skeleton; actin; integrin; Neurospora crassa;

D O I：

10.1006/fgbi.2000.1201

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The apical plasma membrane (PM) is important in hyphal tip growth, where it may regulate tip extensibility via its association with an appropriate membrane skeleton (MS), By cell fractionation and immunocytochemistry we show that proteins with characteristics of actin, spectrin, and integrin are associated in a MS-like manner with the PM of Neurospora crassa hyphae, The spectrin-like protein in particular is highly concentrated at the PM in the region of maximum apical expansion, This protein shares with other spectrins immunoreactivity, molecular weight, PM association, and actin binding capacity, Its distribution in hyphae suggests that it is a dominant component of the MS in true fungi and is critical to hyphal tip growth, (C) 2000 Academic Press.

引用

页码：33 / 44

页数：12

共 97 条

[1] Methods for isolation and analysis of the cytoskeleton [J].