Murine gammaherpesvirus 68 open reading frame 24 is required for late gene expression after DNA replication

被引：37

作者：

Wong, Elaine

Wu, Ting-Ting

Reyes, Nichole

Deng, Hongyu

Sun, Ren

机构：

[1] Univ Calif Los Angeles, David Geffen Sch Med, Dept Mol & Med Pharmacol, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, David Geffen Sch Med, Dent Res Inst, Los Angeles, CA 90095 USA

[3] Chinese Acad Sci, Inst Biophys, Ctr Infect & Immun, Beijing, Peoples R China

来源：

JOURNAL OF VIROLOGY | 2007年 / 81卷 / 12期

关键词：

D O I：

10.1128/JVI.02726-06

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Open reading frame 24 (ORF24) of murine gammaherpesvirus 68 (MHV-68) is conserved among beta- and gammaherpesviruses; however, its function in viral replication has not been defined. Using MHV-68 as a model, we have identified ORF24 as being essential for viral replication. An ORF24-null virus was generated and shown to be defective in late gene expression. Expression of early genes, as well as viral genome replication, was not affected. Furthermore, the defect in late gene expression was likely due to a deficiency in transcription. Thus, we have identified an MHV-68 protein, ORF24, that is essential for the expression of viral late proteins yet dispensable for viral DNA replication.

引用

收藏

页码：6761 / 6764

页数：4

相关论文

共 25 条

[1] Gammaherpesvirus lytic gene expression as characterized by DNA array [J].

Ahn, JW ;

Powell, KL ;

Kellam, P ;

Alber, DG .

JOURNAL OF VIROLOGY, 2002, 76 (12) :6244-6256

[2] Lytic cycle gene regulation of Epstein-Barr virus [J].

Amon, W ;

Binné, UK ;

Bryant, H ;

Jenkins, PJ ;

Karstegl, CE ;

Farrell, PJ .

JOURNAL OF VIROLOGY, 2004, 78 (24) :13460-13469

[3] ORF18 is a transfactor that is essential for late gene transcription of a gammaherpesvirus [J].

Arumugaswami, Vaithilingaraja ;

Wu, Ting-Ting ;

Martinez-Guzman, DeeAnn ;

Jia, Qingmei ;

Deng, Hongyu ;

Reyes, Nichole ;

Sun, Ren .

JOURNAL OF VIROLOGY, 2006, 80 (19) :9730-9740

[4] Identification of proteins associated with murine gammaherpesvirus 68 virions [J].

Bortz, E ;

Whitelegge, JP ;

Jia, QM ;

Zhou, ZH ;

Stewart, JP ;

Wu, TT ;

Sun, R .

JOURNAL OF VIROLOGY, 2003, 77 (24) :13425-13432

[5] On the control of late gene expression in Kaposi's sarcoma-associated herpesvirus (human herpesvirus-8) [J].

Chang, J ;

Ganem, D .

JOURNAL OF GENERAL VIROLOGY, 2000, 81 :2039-2047

[6] Effector CD4(+) and CD8(+) T-cell mechanisms in the control of respiratory virus infections [J].

Doherty, PC ;

Topham, DJ ;

Tripp, RA ;

Cardin, RD ;

Brooks, JW ;

Stevenson, PG .

IMMUNOLOGICAL REVIEWS, 1997, 159 :105-117

[7] Functional profiling of a human cytomegalovirus genome [J].

Dunn, W ;

Chou, C ;

Li, H ;

Hai, R ;

Patterson, D ;

Stolc, V ;

Zhu, H ;

Liu, FY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (24) :14223-14228

[8] Transcriptome profile of murine gammaherpesvirus-68 lytic infection [J].

Ebrahimi, B ;

Dutia, BM ;

Roberts, KL ;

Garcia-Ramirez, JJ ;

Dickinson, P ;

Stewart, JP ;

Ghazal, P ;

Roy, DJ ;

Nash, AA .

JOURNAL OF GENERAL VIROLOGY, 2003, 84 :99-109

[9] POTENTIAL ROLE FOR HERPES-SIMPLEX VIRUS ICP8 DNA-REPLICATION PROTEIN IN STIMULATION OF LATE GENE-EXPRESSION [J].

GAO, M ;

KNIPE, DM .

JOURNAL OF VIROLOGY, 1991, 65 (05) :2666-2675

[10] Murine gammaherpesvirus 68 open reading frame 31 is required for viral replication [J].

Jia, QM ;

Wu, TT ;

Liao, HI ;

Chernishof, V ;

Sun, R .

JOURNAL OF VIROLOGY, 2004, 78 (12) :6610-6620