Thermodynamic Study on the Interaction of Copper Ion with Human Growth Hormone

被引：4

作者：

Behbehani, G. Rezaei ^{[1
,2
]}

Tazikeh, E. ^{[2
]}

Saboury, A. A. ^{[3
]}

Divsalar, A. ^{[3
,4
]}

Monajjemi, M. ^{[2
]}

机构：

[1] Imam Khomeini Int Univ, Dept Chem, Qazvin, Iran

[2] Islamic Azad Univ, Dept Chem, Sci & Res Branch, Tehran, Iran

[3] Univ Tehran, Inst Biochem & Biophys, Tehran, Iran

[4] Tarbiat Moallem Univ, Dept Biol Sci, Tehran, Iran

来源：

JOURNAL OF SOLUTION CHEMISTRY | 2010年 / 39卷 / 02期

关键词：

Isothermal titration calorimetry; Human growth hormone (hGH); Solvation model; DIRECT CALORIMETRIC DETERMINATION; AQUEOUS-METHANOL; DENATURATION ENTHALPY; BINDING; WATER; REPRODUCE; ETHANOL; ACETONITRILE; DIMERIZATION; STABILITY;

D O I：

10.1007/s10953-010-9498-9

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A thermodynamic study on the interaction between the Cu2+ ion and human growth hormone (hGH) was studied at the temperatures 300.15 and 310.15 K in NaCl solution using isothermal titration calorimetry. The new solvation model was used to reproduce the enthalpies of Cu2+ + hGH interaction over the whole range of Cu2+ concentrations. It was found that there is a set of three identical and non-interacting binding sites for Cu2+ ions. The intrinsic dissociation equilibrium constant and the molar enthalpy of binding are 1313.4 mu mol.L-1 and -16.80 kJ.mol(-1) at 300.15 K, and 1648.2 mu mol.L-1 and -16.40 kJ.mol(-1) and 310.15 K, respectively. The binding parameters recovered from the new equation are attributed to a structural change of hGH and its biological activity due to metal ion interaction.

引用

页码：153 / 164

页数：12

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