Expression of mRNA for glucose transport proteins in jejunum, liver, kidney and skeletal muscle of pigs

被引:45
作者
Aschenbach, J. R. [1 ]
Steglich, K. [1 ]
Gaebel, G. [1 ]
Honscha, K. U. [1 ]
机构
[1] Univ Leipzig, Inst Vet Physiol, D-04103 Leipzig, Germany
关键词
Glucose transport; mRNA expression; Sodium-glucose cotransport; Pig; RAT SMALL-INTESTINE; SUGAR-TRANSPORT; DEVELOPMENTAL REGULATION; CACO-2; CELLS; COTRANSPORTER; GLUT8; FRUCTOSE; CLONING; HUMANS; LOCALIZATION;
D O I
10.1007/BF03180578
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
J.R. ASCHENBACH, K. STEGLICH, G. GABEL and K.U. HONSCHA. Expression of mRNA for glucose transport proteins in jejunum, liver, kidney and skeletal muscle of pigs. J Physiol Biochem, 65 (3), 251-266, 2009. Although pigs arc adapted to starch-rich diets and have high turnover rates of glucose, very scat-cc information is available oil the molecular basis of glucose transport. Therefore, the present study attempted a systematic screening for the presence of mRNA of glucose transport proteins in main organs of glucose absorption, production and conservation. From the members of the solute carrier family SLC5A (sodium glucose cotransporter), the porcine jejunum was positive for SGLT1 and SGLT3, but also contained detectable levels of SGLT5. Liver contained SGLT1, SGLT5, traces of SGLT3 and, in one of five pigs, SGLT2. Kidney contained SGLT1, SGLT2, SGLT3, SGLT5 and hardly detectable levels of SGLT4. Skeletal muscle showed weak signals for SGLT3 and SGLT5. Screening for members of the SLC2A family (facilitated glucose transporter) in intestine revealed the presence of mRNA for GLUT1, GLUT2, GLUT5, GLUT7 and GLUTS, while GLUT3, GLUT4, GLUT10 and GLUT11 were also detectable. The liver contained GLUT1, GLUT2 and GLUTS mRNA, while GLUT3, GLUT4, GLUT5, GLUT10 and GLUT11 were poorly detectable. The kidney was positive for GLUT1, GLUT2, GLUT5, GLUTS and GLUT 11, but traces of GLUT3, GLUT4 and GLUT 10 could also be detected. Skeletal muscle had the strongest signal for GLUT4, while GLUT1, GLUT3, GLUT5, GLUTS, GLUT10 and GLUT11 showed weak signals. A total of 12 unique partial cDNA sequences were submitted to GenBank. In conclusion, this study provides molecular insight into the organ-specific expression of glucose transporters in pigs and thus sheds light on the way of glucose handling in this omnivorous species.
引用
收藏
页码:251 / 266
页数:16
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