An on-line electrophoretic concentration method for capillary electrophoresis of proteins

被引:57
作者
Wu, XZ
Hosaka, A
Hobo, T
机构
[1] Fukui Univ, Fac Engn, Dept Mat Sci & Engn, Fukui 910, Japan
[2] Tokyo Metropolitan Univ, Fac Engn, Dept Ind Chem, Hachioji, Tokyo 19203, Japan
关键词
D O I
10.1021/ac9709110
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A simple on-line electrophoretic concentration method for capillary electrophoresis of proteins with a semipermeable hollow fiber is proposed. A short, semipermeable hollow fiber is connected to the inlet end of a capillary. An injection electric field is applied across the hollow fiber. Protein samples are electromigrated into the hollow fiber from a sample vial. Since the protein ions cannot pass through wall of the hollow fiber, protein ions are concentrated in the hollow fiber. After a certain period of injection (concentration) time, an electric field is applied across the hollow fiber and the capillary, and a conventional CE is carried out. The concentration effect of the method has been demonstrated successfully with model protein samples, cytochrome c, lysozyme, ribonuclease A, and alpha-chymotrypsinogen A. Experimental results show that the concentration factor is increased with the injection (concentration) time and the electrophoretic mobility of the proteins. When the injection (concentration) time is 60 s, CE detection limits of the model proteins can be lowered by 1000-fold for the present method. Factors affecting the concentration factors and the further improvements of the method are discussed.
引用
收藏
页码:2081 / 2084
页数:4
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