Protein splicing and its applications

被引：57

作者：

Perler, FB ^{[1
]}

Adam, E ^{[1
]}

机构：

[1] New England Biolabs Inc, Beverly, MA 01915 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2000年 / 11卷 / 04期

关键词：

D O I：

10.1016/S0958-1669(00)00113-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein splicing elements, termed inteins, provide a fertile source for innovative biotechnology tools. First harnessed for protein purification, inteins are now used to express cytotoxic proteins, to segmentally modify or label proteins, to cyclize proteins or peptides, to study structure-activity relationships and to generate reactive polypeptide termini in expressed proteins for an expanding list of chemoselective reactions, including protein ligation.

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页码：377 / 383

页数：7

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