Rapid Structural Identification of Cytotoxic Bufadienolide Sulfates in Toad Venom from Bufo melanosticus by LC-DAD-MSn and LC-SPE-NMR

被引：33

作者：

Gao, Huimin ^{[2
]}

Zehl, Martin ^{[1
]}

Kaehlig, Hanspeter ^{[3
]}

Schneider, Peter ^{[4
]}

Stuppner, Hermann ^{[4
]}

Banuls, Laetitia Moreno Y. ^{[5
]}

Kiss, Robert ^{[5
]}

Kopp, Brigitte ^{[1
]}

机构：

[1] Univ Vienna, Dept Pharmacognosy, A-1090 Vienna, Austria

[2] China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China

[3] Univ Vienna, Inst Organ Chem, A-1090 Vienna, Austria

[4] Univ Innsbruck, Inst Pharm Pharmacognosy, A-6020 Innsbruck, Austria

[5] Univ Libre Bruxelles, Inst Pharm, Toxicol Lab, B-1050 Brussels, Belgium

来源：

JOURNAL OF NATURAL PRODUCTS | 2010年 / 73卷 / 04期

基金：

中国国家自然科学基金;

关键词：

PERFORMANCE LIQUID-CHROMATOGRAPHY; ANTICANCER THERAPY; SODIUM-PUMP; SCHNEIDER; STEROIDS; SKIN; SPECTROSCOPY; METABOLITES; MARINUS; TARGETS;

D O I：

10.1021/np900746k

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Toad venom, namely, "Chansu" in China, has been widely used for the treatment of heart failure, sores, pains, and various cancers. Upon LC-MS analysis of the venom from Bufo melanosticus collected in Indonesia, new bufadienolide sulfates were identified. For a complete characterization, the MeOH extract of the toad venom from B. melanosticus was fractionated by preparative HPLC, and the structures of five new buadienolide sulfates (1-5) along with one new bufogenin (6) were rapidly elucidated on the basis of LC-DAD-MSn and LC-SPE-NMR data. The in vitro growth inhibitory activity of these six compounds along with hellebrin (positive control) has been assayed by means of the MTT colorimetric assay in four human and two mouse cancer cell lines. Compound 3 and hellebrin displayed similar and marked in vitro cytotoxicity.

引用

页码：603 / 608

页数：6

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