Characterisation of Archaea in soils by polar lipid analysis

被引:53
作者
Gattinger, A [1 ]
Günther, A [1 ]
Schloter, M [1 ]
Munich, JC [1 ]
机构
[1] GSF Forschungszentrum Umwelt & Gesundheit, Inst Bodenokol, D-85764 Neuherberg, Germany
来源
ACTA BIOTECHNOLOGICA | 2003年 / 23卷 / 01期
关键词
D O I
10.1002/abio.200390003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
While phospholipid fatty acid (PLFA) profiling is a well-established method used for the determination of bacterial and eukaryotic organisms in soil ecology, phospholipid etherlipid (PLEL) analyses for the characterisation of Archaea is a rather new approach. Analyses of PLEL derived isoprenoid side chains by GC/MS provided a broad picture of the archaeal community in a mixed soil extract, as lipids previously identified in isolates belonging to the kingdoms Eury- and Crenarchaeota were covered. Furthermore, ether-linked isoprenoid hydrocarbons, which have not been detected in archaeal isolates and monomethyl-branched alkanes which have only been found in hyperthermophilic bacteria, were detected in these soil extracts. Monomethyl-branched alkanes were the most dominant ones and accounted for 43.4% of the total identified ether-linked hydrocarbons, followed by straight chain (unbranched) and isoprenoid hydrocarbons, which accounted for 34.6 and 15.5%, respectively.
引用
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页码:21 / 28
页数:8
相关论文
共 18 条
[1]  
Bai Q, 2000, MICROBIAL ECOL, V39, P273
[2]   Direct linking of microbial populations to specific biogeochemical processes by 13C-labelling of biomarkers [J].
Boschker, HTS ;
Nold, SC ;
Wellsbury, P ;
Bos, D ;
de Graaf, W ;
Pel, R ;
Parkes, RJ ;
Cappenberg, TE .
NATURE, 1998, 392 (6678) :801-805
[3]   A NEW 15,16-DIMETHYL-30-GLYCERYLOXYTRIACONTANOIC ACID FROM LIPIDS OF THERMOTOGA-MARITIMA [J].
DEROSA, M ;
GAMBACORTA, A ;
HUBER, R ;
LANZOTTI, V ;
NICOLAUS, B ;
STETTER, KO ;
TRINCONE, A .
JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1988, (19) :1300-1301
[4]   Phospholipid etherlipid and phospholipid fatty acid fingerprints in selected euryarchaeotal monocultures for taxonomic profiling [J].
Gattinger, A ;
Schloter, M ;
Munch, JC .
FEMS MICROBIOLOGY LETTERS, 2002, 213 (01) :133-139
[5]   AQUIFEX-PYROPHILUS GEN-NOV SP-NOV REPRESENTS A NOVEL GROUP OF MARINE HYPERTHERMOPHILIC HYDROGEN-OXIDIZING BACTERIA [J].
HUBER, R ;
WILHARM, T ;
HUBER, D ;
TRINCONE, A ;
BURGGRAF, S ;
KONIG, H ;
RACHEL, R ;
ROCKINGER, I ;
FRICKE, H ;
STETTER, KO .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1992, 15 (03) :340-351
[6]   A DIETHER ANALOG OF PHOSPHATIDYL GLYCEROPHOSPHATE IN HALOBACTERIUM CUTIRUBRUM [J].
KATES, M ;
YENGOYAN, LS ;
SASTRY, PS .
BIOCHIMICA ET BIOPHYSICA ACTA, 1965, 98 (02) :252-+
[7]  
Kates M., 1993, BIOCH ARCHAEA ARCHAE, P261, DOI DOI 10.1016/S0167-7306(08)60258-6
[8]  
LANGWORTHY TA, 1983, SYST APPL MICROBIOL, V4, P1, DOI 10.1016/S0723-2020(83)80029-0
[9]  
Liesack W., 1997, Modern soil microbiology., P375
[10]  
MANCUSO CA, 1985, J LIPID RES, V26, P1120