A mimic as internal standard to monitor PCR analysis of foodborne pathogens

被引：20

作者：

Lambertz, ST

Ballagi-Pordany, A

Lindqvist, R

机构：

[1] Swedish Univ Agr Sci, Natl Food Adm, S-75126 Uppsala, Sweden

[2] Swedish Univ Agr Sci, Fac Vet Med, Dept Vet Microbiol, Sect Virol, S-75126 Uppsala, Sweden

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1998年 / 26卷 / 01期

关键词：

D O I：

10.1046/j.1472-765X.1998.00256.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This report describes a method for the construction of a mimic molecule for monitoring PCR. A blue cheese homogenate spiked with Yersinia enterocolitica was used to demonstrate how false-negative PCR results may be avoided using the mimic. Further, the possibility of estimating bacterial numbers using the mimic was illustrated by varying the target concentration.

引用

收藏

页码：9 / 11

页数：3

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