Sequencing and high level expression in Escherichia coli of the tropomyosin allergen (Der p 10) from Dermatophagoides pteronyssinus

被引：74

作者：

Asturias, JA ^{[1
]}

Arilla, MC ^{[1
]}

Gómez-Bayón, N ^{[1
]}

Martínez, A ^{[1
]}

Martínez, J ^{[1
]}

Palacios, R ^{[1
]}

机构：

[1] IFIDESA, ARISTEGUI, Dept Res & Dev, Bilbao 48008, Spain

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1998年 / 1397卷 / 01期

关键词：

allergy; IgE antibody; pan allergen; recombinant allergen; (dust mite);

D O I：

10.1016/S0167-4781(98)00006-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cDNA encoding an allergen from the dust mite Dermatophagoides pteronyssinus has been cloned and sequenced. The allergen (Der p 10) is a tropomyosin that shared more than 65% identical residues with other invertebrate tropomyosins. The final recovery of recombinant Der p 10 from the culture media after a single purification step was as much as 26 mg/l. The recombinant allergen is reactive to shrimp antitropomyosin IgG antibodies and has a 5.6% frequency of IgE reactivity in sera from mite-allergic patients. (C) 1998 Elsevier Science B.V.

引用

页码：27 / 30

页数：4

共 22 条

[1] IMMUNOCHEMICAL CHARACTERIZATION OF RECOMBINANT AND NATIVE TROPOMYOSINS AS A NEW ALLERGEN FROM THE HOUSE-DUST MITE, DERMATOPHAGOIDES-FARINAE
AKI, T
KODAMA, T
FUJIKAWA, A
MIURA, K
SHIGETA, S
WADA, T
JYO, T
MUROOKA, Y
OKA, S
ONO, K
[J]. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1995, 96 (01) : 74 - 83
[2] Cloning and expression of the panallergen profilin and the major allergen (Ole e 1) from olive tree pollen
Asturias, JA
Arilla, MC
GomezBayon, N
Martinez, J
Martinez, A
Palacios, R
[J]. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1997, 100 (03) : 365 - 372
[3] DETECTION OF IGE ANTIBODIES TO A WIDE-RANGE OF INSECT SPECIES IN SUBJECTS WITH SUSPECTED INHALANT ALLERGIES TO INSECTS
BALDO, BA
PANZANI, RC
[J]. INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1988, 85 (03): : 278 - 287
[4] BUSCH RK, 1996, IMMUNOL ALLERGY CLIN, V16, P535
[5] DEMAATBLEEKER F, 1995, ALLERGY, V50, P438, DOI 10.1111/j.1398-9995.1995.tb01174.x
[6] IgE reactivity against a cross-reactive allergen in crustacea and mollusca: Evidence fev tropomyosin as the common allergen
Leung, PSC
Chow, WK
Duffey, S
Kwan, HS
Gershwin, ME
Chu, KH
[J]. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1996, 98 (05) : 954 - 961
[7] Martinez A, 1997, Allergol Immunopathol (Madr), V25, P118
[8] COMPARISON OF THE LEVELS OF THE MAJOR ALLERGENS DER-P-I AND DER-P-II IN STANDARDIZED EXTRACTS OF THE HOUSE-DUST MITE, DERMATOPHAGOIDES-PTERONYSSINUS
MEYER, CH
BOND, JF
CHEN, MS
KASAIAN, MT
[J]. CLINICAL AND EXPERIMENTAL ALLERGY, 1994, 24 (11) : 1041 - 1048
[9] CLONING AND CHARACTERIZATION OF A MAJOR ALLERGEN OF THE HOUSE-DUST MITE, DERMATOPHAGOIDES-PTERONYSSINUS, HOMOLOGOUS WITH GLUTATHIONE-S-TRANSFERASE
ONEILL, GM
DONOVAN, GR
BALDO, BA
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1994, 1219 (02): : 521 - 528
[10] DUST MITE ALLERGENS AND ASTHMA - REPORT OF A 2ND INTERNATIONAL WORKSHOP
PLATTSMILLS, TAE
THOMAS, WR
AALBERSE, RC
VERVLOET, D
CHAPMAN, MD
BISCHOFF, E
BRUIJNZEELKOOMEN, CAFM
CHARPIN, D
COLLOFF, MJ
EGGLESTON, PA
EHNERT, B
GOLDSTEIN, RA
VANHAGEHAMSTEN, M
HART, BJ
HOLGATE, ST
HONG, CS
JOHANSSON, SGO
KORSGAARD, J
LAU, S
LEMAO, J
LOWENSTEIN, H
MERRETT, TG
MITCHELL, EB
MIYAMOTO, T
MUDDE, GC
NASPITZ, CK
OHEHIR, RE
OKUDAIRA, H
PAULI, G
POLLART, SM
REED, CE
REES, J
RING, J
ROMAGNANI, S
SCHOU, C
SEARS, MR
SPIEKSMA, FTM
STEWART, GA
THOMAS, WR
TOVEY, ER
TURNER, KJ
VERVLOET, D
VIELUF, D
DEWECK, AL
WEN, T
WAHN, U
WOOLCOCK, AJ
YASUEDA, H
YOUNG, R
[J]. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1992, 89 (05) : 1046 - 1060

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