Activation of the myocyte enhancer factor-2 transcription factor by calcium/calmodulin-dependent protein kinase-stimulated binding of 14-3-3 to histone deacetylase 5

被引:404
作者
McKinsey, TA [1 ]
Zhang, CL [1 ]
Olson, EN [1 ]
机构
[1] Univ Texas, SW Med Ctr, Dept Mol Biol, Dallas, TX 75390 USA
关键词
D O I
10.1073/pnas.260501497
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Skeletal muscle differentiation is controlled by interactions between myocyte enhancer factor-2 (MEF2) and myogenic basic helix-loop-helix transcription factors. Association of MEF2 with histone deacetylases (HDAC) -4 and -5 results in repression of MEF2 target genes and inhibition of myogenesis. Calcium/calmodulin-dependent protein kinase (CaMK) signaling promotes myogenesis by disrupting MEF2-HDAC complexes and stimulating HDAC nuclear export. To further define the mechanisms that confer CaMK responsiveness to HDAC4 and -5, we performed yeast two-hybrid screens to identify HDAC-interacting factors. These screens revealed interactions between HDAC4 and members of the 14-3-3 family of proteins, which function as signal-dependent intracellular chaperones. HDAC4 binds constitutively to 14-3-3 in yeast and mammalian cells, whereas HDAC5 binding to 14-3-3 is largely dependent on CaMK signaling. CaMK phosphorylates serines -259 and -498 in HDAC5, which subsequently serve as docking sites for 14-3-3. Our studies suggest that 14-3-3 binding to HDAC5 is required for CaMK-dependent disruption of MEF2-HDAC complexes and nuclear export of HDAC5, and implicate 14-3-3 as a signal-dependent regulator of muscle cell differentiation.
引用
收藏
页码:14400 / 14405
页数:6
相关论文
共 26 条
  • [1] IDENTIFICATION OF THE SITES IN MAP KINASE KINASE-1 PHOSPHORYLATED BY P74(RAF-1)
    ALESSI, DR
    SAITO, Y
    CAMPBELL, DG
    COHEN, P
    SITHANANDAM, G
    RAPP, U
    ASHWORTH, A
    MARSHALL, CJ
    COWLEY, S
    [J]. EMBO JOURNAL, 1994, 13 (07) : 1610 - 1619
  • [2] Transcriptional control of muscle development by myocyte enhancer factor-2 (MEF2) proteins
    Black, BL
    Olson, EN
    [J]. ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1998, 14 : 167 - 196
  • [3] Ca2+-dependent gene expression mediated by MEF2 transcription factors
    Blaeser, F
    Ho, N
    Prywes, R
    Chatila, TA
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (01) : 197 - 209
  • [4] A new family of human histone deacetylases related to Saccharomyces cerevisiae HDA1p
    Fischle, W
    Emiliani, S
    Hendzel, MJ
    Nagase, T
    Nomura, N
    Voelter, W
    Verdin, E
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (17) : 11713 - 11720
  • [5] 14-3-3 proteins: Structure, function, and regulation
    Fu, HA
    Subramanian, RR
    Masters, SC
    [J]. ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 2000, 40 : 617 - 647
  • [6] Regulation of histone deacetylase 4 and 5 and transcriptional activity by 14-3-3-dependent cellular localization
    Grozinger, CM
    Schreiber, SL
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (14) : 7835 - 7840
  • [7] Three proteins define a class of human histone deacetylases related to yeast Hda1p
    Grozinger, CM
    Hassig, CA
    Schreiber, SL
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (09) : 4868 - 4873
  • [8] Activation of the transcription factor MEF2C by the MAP kinase p38 in inflammation
    Han, J
    Jiang, Y
    Li, Z
    Kravchenko, VV
    Ulevitch, RJ
    [J]. NATURE, 1997, 386 (6622) : 296 - 299
  • [9] HUMAN CALCIUM-CALMODULIN DEPENDENT PROTEIN-KINASE-I - CDNA CLONING, DOMAIN-STRUCTURE AND ACTIVATION BY PHOSPHORYLATION AT THREONINE-177 BY CALCIUM-CALMODULIN DEPENDENT PROTEIN-KINASE-I KINASE
    HARIBABU, B
    HOOK, SS
    SELBERT, MA
    GOLDSTEIN, EG
    TOMHAVE, ED
    EDELMAN, AM
    SNYDERMAN, R
    MEANS, AR
    [J]. EMBO JOURNAL, 1995, 14 (15) : 3679 - 3686
  • [10] BMK1/ERK5 regulates serum-induced early gene expression through transcription factor MEF2C
    Kato, Y
    Kravchenko, VV
    Tapping, RI
    Han, JH
    Ulevitch, RJ
    Lee, JD
    [J]. EMBO JOURNAL, 1997, 16 (23) : 7054 - 7066