Diagnosis of avian adenovirus infections using DNA in situ hybridization

被引:18
作者
Latimer, KS [1 ]
Niagro, FD
Williams, OC
Ramis, A
Goodwin, MA
Ritchie, BW
Campagnoli, RP
机构
[1] Univ Georgia, Coll Vet Med, Dept Vet Pathol, Athens, GA 30605 USA
[2] Tuskegee Univ, Sch Vet Med, Tuskegee, AL 36088 USA
[3] Univ Autonoma Barcelona, Fac Vet, E-08193 Barcelona, Spain
[4] Georgia Poultry Lab, Oakwood, GA 30566 USA
关键词
avian adenovirus; aviadenovirus; in situ hybridization; chicken; psittaciform birds; polyomavirus; herpesvirus; Pacheco's parrot disease;
D O I
10.2307/1592329
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Three DNA oligonucleotide probes designated FN-23, FN-48, and FN-96 were evaluated for the diagnosis of aviadenovirus infections by DNA in situ hybridization. Paraffin-embedded tissues were obtained from birds with confirmed adenovirus infection, birds with putative adenovirus infections, and birds with intranuclear inclusions caused by herpesvirus and polyomavirus. In birds with confirmed adenovirus infection, probes FN-23 and FN-96 identified 78% and 72% of diseased individuals, respectively. Only probe FN-48 detected chickens with group II adenovirus infection. In birds with putative adenovirus infection, the DNA probes confirmed aviadenovirus infections in 76% of the population. Probes FN-23, FN-96, and FN-48 detected 85%, 74%, and 18% of adenovirus-infected birds, respectively. None of the DNA probes cross-hybridized with tissues from polyomavirus-infected psittaciform birds or with tissues from a chicken with infectious laryngotracheitis. In contrast, probe FN-23 did cross-hybridize to herpesvirus-infected tissues from two of eight psittaciform birds with Pacheco's parrot disease. Probes FN-48 and FN-96 did not react with these tissues.
引用
收藏
页码:773 / 782
页数:10
相关论文
共 31 条
[1]   INSITU HYBRIDIZATION - AN OPTIMIZED DETECTION PROTOCOL FOR A BIOTINYLATED DNA PROBE RENDERS IT MORE SENSITIVE THAN A COMPARABLE S-35-LABELED PROBE [J].
ALLAN, GM ;
TODD, D ;
SMYTH, JA ;
MACKIE, DP ;
BURNS, J ;
MCNULTY, MS .
JOURNAL OF VIROLOGICAL METHODS, 1989, 24 (1-2) :181-190
[2]   OUTBREAKS OF EGG DROP SYNDROME DUE TO EDS-76 VIRUS IN QUAIL (COTURNIX-COTURNIX-JAPONICA) [J].
DAS, BB ;
PRADHAN, HK .
VETERINARY RECORD, 1992, 131 (12) :264-265
[3]   RESPIRATORY ADENOVIRUS-LIKE INFECTION IN A ROSE-RINGED PARAKEET (PSITTACULA-KRAMERI) [J].
DESMIDT, M ;
DUCATELLE, R ;
UYTTEBROEK, E ;
CHARLIER, G ;
HOORENS, J .
AVIAN DISEASES, 1991, 35 (04) :1001-1006
[4]   INCLUSION-BODY HEPATITIS AND HEMORRHAGIC ENTERITIS IN 2 AFRICAN GREY PARROTS (PSITTACUS-ERITHACUS) ASSOCIATED WITH ADENOVIRUS [J].
DROUAL, R ;
WOOLCOCK, PR ;
NORDHAUSEN, RW ;
FITZGERALD, SD .
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1995, 7 (01) :150-154
[5]  
FENNER F, 1987, VET VIROLOGY, P329
[6]   DETECTION OF TYPE-II AVIAN ADENOVIRAL ANTIGEN IN TISSUE-SECTIONS USING IMMUNOHISTOCHEMICAL STAINING [J].
FITZGERALD, SD ;
REED, WM ;
BURNSTEIN, T .
AVIAN DISEASES, 1992, 36 (02) :341-347
[7]   A REVIEW OF MARBLE SPLEEN DISEASE OF RING-NECKED PHEASANTS [J].
FITZGERALD, SD ;
REED, WM .
JOURNAL OF WILDLIFE DISEASES, 1989, 25 (04) :455-461
[8]   A RETROSPECTIVE IMMUNOHISTOCHEMICAL STUDY OF TYPE-II AVIAN ADENOVIRAL INFECTION IN TURKEY, PHEASANT, AND CHICKEN TISSUES [J].
FITZGERALD, SD ;
REED, WM ;
LANGHEINRICH, KA ;
PORTER, AS ;
LUMBERT, LA .
AVIAN DISEASES, 1994, 38 (01) :78-85
[9]  
GERLACH H, 1986, CLIN AVIAN MED SURG, P428
[10]  
Goodwin M. A., 1992, Journal of the Association of Avian Veterinarians, V6, P37, DOI 10.2307/30136928