A novel method for the rational construction of well-defined immunogens: The use of oximation to conjugate cholera toxin B subunit to a peptide-polyoxime complex

被引:29
作者
Chen, JH
Zeng, WG
Offord, R
Rose, K
机构
[1] Univ Geneva, Ctr Med, Dept Med Biochem, CH-1211 Geneva, Switzerland
[2] Univ Melbourne, Dept Microbiol & Immunol, Cooperat Res Ctr Vaccine Technol, Parkville, Vic 3052, Australia
关键词
D O I
10.1021/bc025651u
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Cholera toxin B subunit (CTB), capable of binding to all mucous membranes in its pentameric form, is a potential carrier of mucosal vaccines. In our previous work we reported that the N-terminus of CTB, a threonine, could in principle undergo oxidation and oximation to form conjugates with a cascade of immunogenic peptides. In this study, we set up a model by chemically coupling CTB to a polyoxime that possessed five copies of influenza virus-derived peptides displayed in comblike form. The construct was reconstituted into pentameric form when eluted from a Superdex column after conjugation, and the pentameric nature of this CTB-viral peptide complex was confirmed by SDS-PAGE. GM(1)-ELISA assay showed that the binding properties of CTB-viral peptide complex were increased 4-5-fold over native CTB.
引用
收藏
页码:614 / 618
页数:5
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