Selective stimulation of translation of leaderless mRNA by initiation factor 2:: evolutionary implications for translation

被引:107
作者
Grill, S
Gualerzi, CO
Londei, P
Bläsi, U
机构
[1] Univ Vienna, Vienna Bioctr, Inst Microbiol & Genet, A-1090 Vienna, Austria
[2] Univ Camerino, Dept Biol, Genet Lab, I-62032 Camerino, Italy
[3] Univ Bari, Dept Med Biochem & Med Biol, I-70124 Bari, Italy
[4] Univ Rome, Dept Cellular Biotechnol & Hematol, I-00161 Rome, Italy
关键词
evolution; IF2; initiator tRNA; leaderless mRNA; translation initiation;
D O I
10.1093/emboj/19.15.4101
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Translation initiation in bacteria involves a stochastic binding mechanism in which the 30S ribosomal subunit first binds either to mRNA or to initiator tRNA, fMet-tRNA(f)(Met). Leaderless lambda cI mRNA did not form a binary complex with 30S ribosomes, which argues against the view that ribosomal recruitment signals other than a 5'-terminal start codon are essential for translation initiation of these mRNAs, We show that, in Escherichia coli, translation initiation factor 2 (IF2) selectively stimulates translation of lambda cI mRNA in vivo and in vitro, These experiments suggest that the start codon of leaderless mRNAs is recognized by a 30S-fMet-tRNA(f)(Met)-IF2 complex, an intermediate equivalent to that obligatorily formed during translation initiation in eukaryotes. We further show that leaderless lambda cI mRNA is faithfully translated in vitro in both archaebacterial and eukaryotic translation systems. This suggests that translation of leaderless mRNAs reflects a fundamental capability of the translational apparatus of all three domains of life and lends support to the hypothesis that the translation initiation pathway is universally conserved.
引用
收藏
页码:4101 / 4110
页数:10
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