Remorins form a novel family of coiled coil-forming oligomeric and filamentous proteins associated with apical, vascular and embryonic tissues in plants

被引:68
作者
Bariola, PA
Retelska, D
Stasiak, A
Kammerer, RA
Fleming, A
Hijri, M
Franks, S
Farmer, EE [1 ]
机构
[1] Univ Lausanne, Dept Plant Mol Biol, CH-1015 Lausanne, Switzerland
[2] ID Biomed Washington, Bothell, WA 98011 USA
[3] Univ Lausanne, Lab Ultrastruct Anal, CH-1015 Lausanne, Switzerland
[4] Univ Manchester, Sch Biol Sci, Wellcome Trust Ctr Cell Matrix Res, Manchester M13 9PT, Lancs, England
[5] Swiss Fed Inst Technol, Inst Plant Sci, CH-8092 Zurich, Switzerland
[6] Univ Sheffield, Dept Anim & Plant Sci, Sheffield S10 2TN, S Yorkshire, England
[7] Univ Lausanne, Dept Ecol & Evolut, CH-1015 Lausanne, Switzerland
[8] Univ Basel, Biozentrum, Dept Biophys Chem, CH-4056 Basel, Switzerland
关键词
coiled coil; crosslinking; electron microscopy; filamentous protein; plant;
D O I
10.1007/s11103-004-1520-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Remorins form a superfamily of plant-specific plasma membrane/lipid-raft-associated proteins of unknown structure and function. Using specific antibodies, we localized tomato remorin 1 to apical tissues, leaf primordia and vascular traces. The deduced remorin protein sequence contains a predicted coiled coil-domain, suggesting its participation in protein-protein interactions. Circular dichroisim revealed that recombinant potato remorin contains an alpha-helical region that forms a functional coiled-coil domain. Electron microscopy of purified preparations of four different recombinant remorins, one from potato, two divergent isologs from tomato, and one from Arahidopsis thaliana, demonstrated that the proteins form highly similar filamentous structures. The diameters of the negatively-stained filaments ranged from 4.6-7.4 nm for potato remorin 1, 4.3-6.2 nm for tomato remorin 1, 5.7-7.5 nm for tomato remorin 2, and 5.7-8.0 nm for Arabidopsis Dbp. Highly polymerized remorin 1 was detected in glutaraldehyde-crosslinked tomato plasma membrane preparations and a population of the protein was immunolocalized in tomato root tips to structures associated with discrete regions of the plasma membrane.
引用
收藏
页码:579 / 594
页数:16
相关论文
共 31 条
[1]   AN AUXIN-REGULATED GENE OF ARABIDOPSIS-THALIANA ENCODES A DNA-BINDING PROTEIN [J].
ALLIOTTE, T ;
TIRE, C ;
ENGLER, G ;
PELEMAN, J ;
CAPLAN, A ;
VANMONTAGU, M ;
INZE, D .
PLANT PHYSIOLOGY, 1989, 89 (03) :743-752
[2]   Inhibitors of protein kinases and phosphatases alter root morphology and disorganize cortical microtubules [J].
Baskin, TI ;
Wilson, JE .
PLANT PHYSIOLOGY, 1997, 113 (02) :493-502
[3]  
BENNETT V, 1993, ANNU REV CELL BIOL, V9, P27, DOI 10.1146/annurev.cb.09.110193.000331
[4]   DETERMINATION OF HELIX AND BETA-FORM OF PROTEINS IN AQUEOUS-SOLUTION BY CIRCULAR-DICHROISM [J].
CHEN, YH ;
YANG, JT ;
CHAU, KH .
BIOCHEMISTRY, 1974, 13 (16) :3350-3359
[5]   Improved green fluorescent protein by molecular evolution using DNA shuffling [J].
Crameri, A ;
Whitehorn, EA ;
Tate, E ;
Stemmer, WPC .
NATURE BIOTECHNOLOGY, 1996, 14 (03) :315-319
[6]   Novel components of the plant cytoskeleton: a beginning to plant 'cytomics' [J].
Davies, E ;
Stankovic, B ;
Azama, K ;
Shibata, K ;
Abe, S .
PLANT SCIENCE, 2001, 160 (02) :185-196
[7]   SPECTROSCOPIC DETERMINATION OF TRYPTOPHAN AND TYROSINE IN PROTEINS [J].
EDELHOCH, H .
BIOCHEMISTRY, 1967, 6 (07) :1948-&
[8]  
FARMER EE, 1991, J BIOL CHEM, V266, P3140
[9]   A structural scaffolding of intermediate filaments in health and disease [J].
Fuchs, E ;
Cleveland, DW .
SCIENCE, 1998, 279 (5350) :514-519
[10]   Putative microtubule-associated proteins from the Arabidopsis genome [J].
Gardiner, J ;
Marc, J .
PROTOPLASMA, 2003, 222 (1-2) :61-74