Determination of flunitrazepam and its main metabolites in serum and urine by HPLC after mixed-mode solid-phase extraction

被引：20

作者：

Deinl, I ^{[1
]}

Mahr, G

von Meyer, L

机构：

[1] Friedrich Alexander Univ, Inst Legal Med, D-91054 Erlangen, Germany

[2] Ludwigs Maximillian Univ, Inst Legal Med, D-80337 Munich, Germany

来源：

JOURNAL OF ANALYTICAL TOXICOLOGY | 1998年 / 22卷 / 03期

关键词：

D O I：

10.1093/jat/22.3.197

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid and sensitive method is presented for the simultaneous determination of flunitrazepam, norflunitrazepam, 7-aminoflunitrazepam, and 7-acetamidoflunitrazepam in serum, plasma, and urine. The compounds were extracted by a mixed-mode solid-phase procedure following analysis by high- performance liquid chromatography and ultraviolet detection and using methylclonazepam as the internal standard. The method revealed high recoveries and showed good precision and linearity for all compounds. The limit of detection was at least 1 ng/mL serum (plasma) for all compounds.

引用

页码：197 / 202

页数：6

共 27 条

[1]

AMREIN R, 1978, KLIN ANASTHESIOLOGIE

[2]

BECK O, 1992, CLIN CHEM, V38, P271

[3] SIMULTANEOUS HPLC ANALYSIS OF THE HYPNOTIC BENZODIAZEPINES NITRAZEPAM, ESTAZOLAM, FLUNITRAZEPAM, AND TRIAZOLAM IN PLASMA [J].

BOUKHABZA, A ;

LUGNIER, AAJ ;

KINTZ, P ;

MANGIN, P .

JOURNAL OF ANALYTICAL TOXICOLOGY, 1991, 15 (06) :319-322

[4] PHARMACOKINETICS OF FLUNITRAZEPAM FOLLOWING SINGLE-DOSE AND MULTIPLE-DOSE ORAL-ADMINISTRATION TO HEALTHY HUMAN SUBJECTS [J].

BOXENBAUM, HG ;

POSMANTER, HN ;

MACASIEB, T ;

GEITNER, KA ;

WEINFELD, RE ;

MOORE, JD ;

DARRAGH, A ;

OKELLY, DA ;

WEISSMAN, L ;

KAPLAN, SA .

JOURNAL OF PHARMACOKINETICS AND BIOPHARMACEUTICS, 1978, 6 (04) :283-293

[5] SOLID-PHASE EXTRACTION FOR THE SCREENING OF ACIDIC, NEUTRAL AND BASIC DRUGS IN PLASMA USING A SINGLE-COLUMN PROCEDURE ON BOND ELUT CERTIFY [J].

CHEN, XO ;

WIJSBEEK, J ;

VANVEEN, J ;

FRANKE, JP ;

DEZEEUW, RA .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1990, 529 (01) :161-166

[6]

Daldrup T., 1982, CHROMATOGR NEWSL, V10, P1

[7]

DEINL I, 1996, THESIS LUDWIG MAXIMI

[8] QUANTITATIVE GAS-CHROMATOGRAPHIC ANALYSIS OF FLUNITRAZEPAM IN HUMAN-SERUM WITH ELECTRON-CAPTURE DETECTION [J].

FABER, DB ;

KOK, RM ;

REMPTVANDIJK, EM .

JOURNAL OF CHROMATOGRAPHY, 1977, 133 (02) :319-326

[9] SIMULTANEOUS HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY OF DROPERIDOL AND FLUNITRAZEPAM IN HUMAN PLASMA - APPLICATION TO HEMODILUTION BLOOD-SAMPLES COLLECTED DURING CLINICAL ANESTHESIA [J].

GUICHARD, J ;

PANTEIX, G ;

DUBOST, J ;

BALTASSAT, P ;

ROCHE, C .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1993, 612 (02) :269-275

[10]

HAEFELFINGER P, 1979, HRC-J HIGH RES CHROM, V2, P39

← 1 2 3 →