Applying an excessive mechanical stress alters the effect of subchondral osteoblasts on chondrocytes in a co-culture system

被引：46

作者：

Lin, Yu-Yu ^{[1
]}

Tanaka, Nobuaki ^{[1
]}

Ohkuma, Satoru ^{[1
]}

Iwabuchi, Yasunori ^{[1
]}

Tanne, Yuki ^{[1
]}

Kamiya, Takashi ^{[1
]}

Kunimatsu, Ryo ^{[1
]}

Huang, Yu-Ching ^{[1
]}

Yoshioka, Motoko ^{[1
]}

Mitsuyoshi, Tomomi ^{[1
]}

Tanimoto, Kotaro ^{[1
]}

Tanaka, Eiji ^{[1
]}

Tanne, Kazuo ^{[1
]}

机构：

[1] Hiroshima Univ, Grad Sch Biomed Sci, Dept Orthodont & Craniofacial Dev Biol, Minami Ku, Hiroshima 7348553, Japan

来源：

EUROPEAN JOURNAL OF ORAL SCIENCES | 2010年 / 118卷 / 02期

关键词：

cyclic excessive stress; osteoarthritis; subchondral bone; temporomandibular joint; PORCINE TEMPOROMANDIBULAR-JOINT; MATRIX METALLOPROTEINASES; CULTURED CHONDROCYTES; BONE; OSTEOARTHRITIS; CARTILAGE; DISC; METABOLISM; EXPRESSION; SIALOPROTEIN;

D O I：

10.1111/j.1600-0722.2010.00710.x

中图分类号：

R78 [口腔科学];

学科分类号：

100302 [口腔临床医学];

摘要：

Osteoarthritis (OA) sometimes occurs as a consequence of repeated microtrauma involved in parafunction, which may lead to microfracture in the subchondral bone. The aim of this in vitro study was to evaluate the effects of subchondral osteoblasts in loading with repeated excessive mechanical stress on the metabolism of overlying chondrocytes. A high-magnitude cyclic tensile stress of 15 kPa (30 cycles min-1) was applied to the cultured osteoblasts obtained from porcine mandibular condyles. The chondrocytes in alginate beads were then co-cultured with mechanically stressed or unstressed osteoblasts. Chondrocytes co-cultured with unstressed osteoblasts showed a phenotypic shift to hypertrophic chondrocytes, characterized by decreased expression of type II collagen, aggrecan, Sry-related HMG box (SOX-9), and cartilage oligomeric matrix protein (COMP) genes and increased expression of type X collagen and bone sialoprotein (BSP) genes, suggesting that the co-culture may change the chondrocyte differentiation to some extent. These changes were more distinct in chondrocytes co-cultured with excessively mechanically stressed osteoblasts. After co-culture with stressed osteoblasts, the expressions of matrix metalloproteinase (MMP)1, MMP3 and MMP13 genes were also enhanced and the synthesis of DNA, proteoglycan and collagen were significantly decreased in chondrocytes. These results demonstrate that alterations in cartilage metabolism can be induced by stressed osteoblasts, indicating a possible explanation for the onset and progression of OA.

引用

页码：151 / 158

页数：8

共 39 条

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