Current state and perspectives of fungal DNA barcoding and rapid identification procedures

被引:291
作者
Begerow, Dominik [1 ]
Nilsson, Henrik [2 ,3 ]
Unterseher, Martin [4 ]
Maier, Wolfgang [1 ]
机构
[1] Ruhr Univ Bochum, D-44801 Bochum, Germany
[2] Univ Gothenburg, Dept Plant & Environm Sci, S-40530 Gothenburg, Sweden
[3] Univ Tartu, Inst Ecol & Earth Sci, Dept Bot, EE-51005 Tartu, Estonia
[4] Univ Greifswald, Inst Bot & Landschaftskol, Lehrstuhl Allgemeine & Spezielle Bot, D-17487 Greifswald, Germany
关键词
Fungi; Barcode; ITS rDNA; Taxonomy; Next generation sequencing; ARBUSCULAR MYCORRHIZAL FUNGI; INTERNAL TRANSCRIBED SPACER; MOLECULAR-IDENTIFICATION; SEQUENCE DATABASES; KINGDOM FUNGI; RIBOSOMAL DNA; PCR ASSAY; DIVERSITY; TAXONOMY; FOREST;
D O I
10.1007/s00253-010-2585-4
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Fungal research is experiencing a new wave of methodological improvements that most probably will boost mycology as profoundly as molecular phylogeny has done during the last 15 years. Especially the next generation sequencing technologies can be expected to have a tremendous effect on fungal biodiversity and ecology research. In order to realise the full potential of these exciting techniques by accelerating biodiversity assessments, identification procedures of fungi need to be adapted to the emerging demands of modern large-scale ecological studies. But how should fungal species be identified in the near future? While the answer might seem trivial to most microbiologists, taxonomists working with fungi may have other views. In the present review, we will analyse the state of the art of the so-called barcoding initiatives in the light of fungi, and we will seek to evaluate emerging trends in the field. We will furthermore demonstrate that the usability of DNA barcoding as a major tool for identification of fungi largely depends on the development of high-quality sequence databases that are thoroughly curated by taxonomists and systematists.
引用
收藏
页码:99 / 108
页数:10
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