Biocompatible conductive architecture of carbon nanofiber-doped chitosan prepared with controllable electrodeposition for cytosensing

被引:177
作者
Hao, Chen
Ding, Lin
Zhang, Xueji
Ju, Huangxian [1 ]
机构
[1] Nanjing Univ, Dept Chem, MOE Key Lab Analyt Chem Life Sci, Nanjing 210093, Peoples R China
[2] Univ S Florida, Dept Chem, Tampa, FL 33620 USA
关键词
D O I
10.1021/ac062344z
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A novel architecture was designed by combining the biocompatibility of chitosan (CS) and excellent conductivity of carbon nanofiber (CNF). The controllable electrodeposition of soluble CNF-doped CS colloidal solution formed a robust CNF-CS nanocomposite film with good biocompatibility for the immobilization and cytosensing of K562 cells on an electrode. The formed architecture was characterized using scanning electron microscopic, infrared spectrum, contact angle, and thermogravimetric analyses. The adhesion of K562 cells on the nanocomposite film-modified electrode could be followed with electrochemical impedance spectroscopy and cyclic voltammetry. The presence of CNF facilitated the electrochemical behavior of K562 cells. The impedance of electronic transduction was related to the amount of the adhered cells, producing a highly sensitive impedance sensor for K562 cells ranging from 5 x 10(3) to 5.0 x 10(7) cells mL(-1) with a limit of detection of 1 x 10(3) cells mL(-1). This work suggested a strategy to prepare a biocompatible and conductive interface for immobilization and electrochemical detection of cells and opened a way for the application of CNF in cytosensing.
引用
收藏
页码:4442 / 4447
页数:6
相关论文
共 40 条
[1]   Blood protein adsorption onto chitosan [J].
Benesch, J ;
Tengvall, P .
BIOMATERIALS, 2002, 23 (12) :2561-2568
[2]   Cobalt on carbon nanofiber catalysts: auspicious system for study of manganese promotion in Fischer-Tropsch catalysis [J].
Bezemer, GL ;
Falke, U ;
van Dillen, AJ ;
de Jong, KP .
CHEMICAL COMMUNICATIONS, 2005, (06) :731-733
[3]   Electrochemical antitumor drug sensitivity test for leukemia K562 cells at a carbon-nanotube-modified electrode [J].
Chen, J ;
Du, D ;
Yan, F ;
Ju, HM ;
Lian, HZ .
CHEMISTRY-A EUROPEAN JOURNAL, 2005, 11 (05) :1467-1472
[4]   Growth of carbon nanofibers on tipless cantilevers for high resolution topography and magnetic force imaging [J].
Cui, H ;
Kalinin, SV ;
Yang, X ;
Lowndes, DH .
NANO LETTERS, 2004, 4 (11) :2157-2161
[5]   Electrochemically induced deposition of a polysaccharide hydrogel onto a patterned surface [J].
Fernandes, R ;
Wu, LQ ;
Chen, TH ;
Yi, HM ;
Rubloff, GW ;
Ghodssi, R ;
Bentley, WE ;
Payne, GF .
LANGMUIR, 2003, 19 (10) :4058-4062
[6]   Detection of Escherichia coli O157:H7 using a surface plasmon resonance biosensor [J].
Fratamico, PM ;
Strobaugh, TP ;
Medina, MB ;
Gehring, AG .
BIOTECHNOLOGY TECHNIQUES, 1998, 12 (07) :571-576
[7]   Carbon nanofiber-based active layers for fuel cell cathodes -: preparation and characterization [J].
Hacker, V ;
Wallnöfer, E ;
Baumgartner, W ;
Schaffer, T ;
Besenhard, JO ;
Schröttner, H ;
Schmied, M .
ELECTROCHEMISTRY COMMUNICATIONS, 2005, 7 (04) :377-382
[8]   Fabrication and characterization of polyaniline coated carbon nanofiber for supercapacitor [J].
Jang, J ;
Bae, J ;
Choi, M ;
Yoon, SH .
CARBON, 2005, 43 (13) :2730-2736
[9]   Selective attachment of gold nanoparticles to nitrogen-doped carbon nanotubes [J].
Jiang, KY ;
Eitan, A ;
Schadler, LS ;
Ajayan, PM ;
Siegel, RW ;
Grobert, N ;
Mayne, M ;
Reyes-Reyes, M ;
Terrones, H ;
Terrones, M .
NANO LETTERS, 2003, 3 (03) :275-277
[10]   Microelectrochemical approach to induce local cell adhesion and growth on substrates [J].
Kaji, H ;
Kanada, M ;
Oyamatsu, D ;
Matsue, T ;
Nishizawa, M .
LANGMUIR, 2004, 20 (01) :16-19