Differential gene expression in nasopharyngeal carcinoma cells

被引:91
作者
Fung, LF
Lo, AKF
Yuen, PW
Liu, Y
Wang, XH
Tsao, SW
机构
[1] Univ Hong Kong, Fac Med, Dept Anat, Hong Kong, Hong Kong, Peoples R China
[2] Univ Hong Kong, Fac Med, Dept Surg, Hong Kong, Hong Kong, Peoples R China
关键词
nasopharyngeal carcinoma; cDNA expression array; differential gene expression; calgranulin A; calgranulin B; FRA-1; ENA-78; NF90;
D O I
10.1016/S0024-3205(00)00684-6
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Nasopharyngeal carcinoma (NPC) is a common cancer among southern Chinese. The profile of gene expression in NPC cells is largely unknown. In this study, we have examined differential gene expression in non-malignant and malignant nasopharyngeal epithelial (NPE) cells using a cDNA array hybridization method. A total of 42 genes were identified to be expressed in either non-malignant and malignant NPE cells or both. Thirteen of these genes were overexpressed in malignant NPE cells. These includes nuclear factor (NF90), FOS-related antigen 1 (FRA-1), cytoplasmic dynein light chain (HDLC1), replication factor C (RFC1), nucleoside diphosphate kinase B, UV excision repair protein (RAD23A), insulin-like growth factor receptor II, transcription initiation factor TFIID subunit (TAFII31), growth factor receptor-bound protein 2 (GRB2), UV excision repair protein (RAD23B), glutathione peroxidase, Y box binding protein 1 and heat shock protein 86. In contrast, expression of nine genes was suppressed in malignant NPE cells. These includes calgranulin A, calgranulin B, neutrophil activating protein (ENA-78), heat shock protein 27, integrin beta-1, integrin beta-4, cyclin-dependent kinase inhibitor 1A (p21), interleukin-8 and tyrosine protein kinase receptor (RET). Differential expression of calgranulin A, calgraunlin B, ENA-78, FRA-1 and NF90 in non-malignant and malignant nasophalyngeal epithelial cells was confirmed by RT-PCR analysis. (C) 2000 Elsevier Science Inc.
引用
收藏
页码:923 / 936
页数:14
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