Simultaneously monitoring gene expression kinetics and genetic noise in single cells by optical well arrays

被引：48

作者：

Kuang, Y ^{[1
]}

Biran, I ^{[1
]}

Walt, DR ^{[1
]}

机构：

[1] Tufts Univ, Dept Chem, Medford, MA 02155 USA

来源：

ANALYTICAL CHEMISTRY | 2004年 / 76卷 / 21期

关键词：

D O I：

10.1021/ac049053f

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An optical fiber based well array platform was used for simultaneous, dynamic gene expression monitoring from hundreds of individual live Escherichia coli cells carrying promoter-fluorescent reporter gene fusions. High information content about gene expression kinetics and cell-to-cell gene expression variability can be collected from a single experiment. These data are invaluable for investigating gene regulation and gene networks as well as for systems biology applications.

引用

收藏

页码：6282 / 6286

页数：5

相关论文

共 25 条

[1] Heterogeneity of stress gene expression and stress resistance among individual cells of Saccharomyces cerevisiae [J].

Attfield, PV ;

Choi, HY ;

Veal, DA ;

Bell, PJL .

MOLECULAR MICROBIOLOGY, 2001, 40 (04) :1000-1008

[2] Engineering stability in gene networks by autoregulation [J].

Becskei, A ;

Serrano, L .

NATURE, 2000, 405 (6786) :590-593

[3] Optical imaging fiber-based live bacterial cell array biosensor [J].

Biran, I ;

Rissin, DM ;

Ron, EZ ;

Walt, DR .

ANALYTICAL BIOCHEMISTRY, 2003, 315 (01) :106-113

[4] Optical Imaging fiber-based single live cell arrays: A high-density cell assay platform [J].

Biran, I ;

Walt, DR .

ANALYTICAL CHEMISTRY, 2002, 74 (13) :3046-3054

[5] Noise in eukaryotic gene expression [J].

Blake, WJ ;

Kærn, M ;

Cantor, CR ;

Collins, JJ .

NATURE, 2003, 422 (6932) :633-637

[6] An ultrasensitive bacterial motor revealed by monitoring signaling proteins in single cells [J].

Cluzel, P ;

Surette, M ;

Leibler, S .

SCIENCE, 2000, 287 (5458) :1652-1655

[7] Stochastic gene expression in a single cell [J].

Elowitz, MB ;

Levine, AJ ;

Siggia, ED ;

Swain, PS .

SCIENCE, 2002, 297 (5584) :1183-1186

[8] A synthetic oscillatory network of transcriptional regulators [J].

Elowitz, MB ;

Leibler, S .

NATURE, 2000, 403 (6767) :335-338

[9] The biochemical basis of an all-or-none cell fate switch in Xenopus oocytes [J].

Ferrell, JE ;

Machleder, EM .

SCIENCE, 1998, 280 (5365) :895-898

[10] Construction of a genetic toggle switch in Escherichia coli [J].

Gardner, TS ;

Cantor, CR ;

Collins, JJ .

NATURE, 2000, 403 (6767) :339-342