Regulation of elongating RNA polymerase II by forkhead transcription factors in yeast

被引：58

作者：

Morillon, A

O'Sullivan, J

Azad, A

Proudfoot, N

Mellor, J

机构：

[1] Univ Oxford, Dept Biochem, Oxford OX1 3QU, England

[2] Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England

来源：

SCIENCE | 2003年 / 300卷 / 5618期

关键词：

D O I：

10.1126/science.1081379

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The elongation phase of transcription by RNA polymerase II (RNAPII) is highly regulated and tightly linked to pre-messenger RNA (pre-mRNA) processing. Recent studies have implicated an early elongation checkpoint that facilitates the link to pre-mRNA processing. Here we show that the yeast forkhead transcription factors, Fkh1p and Fkh2p, associate with the coding regions of active genes and influence, in opposing ways, transcriptional elongation and termination. These events are coordinated with serine-5 and -2 phosphorylation of the heptad repeat of the carboxy-terminal domain (CTD) of RNAPII. Our results suggest that, in addition to their documented promoter function, Fkh1p and Fkh2p coordinate early transcription elongation and pre-mRNA processing. This may reflect a general feature of gene regulation in eukaryotes.

引用

收藏

页码：492 / 495

页数：6

相关论文

共 27 条

[1] Forkhead transcription factors: Key players in development and metabolism [J].

Carlsson, P ;

Mahlapuu, M .

DEVELOPMENTAL BIOLOGY, 2002, 250 (01) :1-23

[2] Remodeling of yeast genome expression in response to environmental changes [J].

Causton, HC ;

Ren, B ;

Koh, SS ;

Harbison, CT ;

Kanin, E ;

Jennings, EG ;

Lee, TI ;

True, HL ;

Lander, ES ;

Young, RA .

MOLECULAR BIOLOGY OF THE CELL, 2001, 12 (02) :323-337

[3] Opposing effects of Ctk1 kinase and Fcp1 phosphatase at Ser 2 of the RNA polymerase II C-terminal domain [J].

Cho, EJ ;

Kobor, MS ;

Kim, M ;

Greenblatt, J ;

Buratowski, S .

GENES & DEVELOPMENT, 2001, 15 (24) :3319-3329

[4] Binding of the winged-helix transcription factor HNF3 to a linker histone site on the nucleosome [J].

Cirillo, LA ;

McPherson, CE ;

Bossard, P ;

Stevens, K ;

Cherian, S ;

Shim, EY ;

Clark, KL ;

Burley, SK ;

Zaret, KS .

EMBO JOURNAL, 1998, 17 (01) :244-254

[5] CO-CRYSTAL STRUCTURE OF THE HNF-3/FORK HEAD DNA-RECOGNITION MOTIF RESEMBLES HISTONE-H5 [J].

CLARK, KL ;

HALAY, ED ;

LAI, ES ;

BURLEY, SK .

NATURE, 1993, 364 (6436) :412-420

[6] Terminal exon definition occurs contrascriptionally and promotes termination of RNA polymerase II [J].

Dye, MJ ;

Proudfoot, NJ .

MOLECULAR CELL, 1999, 3 (03) :371-378

[7] 6-AZAURACIL INHIBITION OF GTP BIOSYNTHESIS IN SACCHAROMYCES-CEREVISIAE [J].

EXINGER, F ;

LACROUTE, F .

CURRENT GENETICS, 1992, 22 (01) :9-11

[8] Promoting elongation with transcript cleavage stimulatory factors [J].

Fish, RN ;

Kane, CM .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2002, 1577 (02) :287-307

[9] Genomic expression programs in the response of yeast cells to environmental changes [J].

Gasch, AP ;

Spellman, PT ;

Kao, CM ;

Carmel-Harel, O ;

Eisen, MB ;

Storz, G ;

Botstein, D ;

Brown, PO .

MOLECULAR BIOLOGY OF THE CELL, 2000, 11 (12) :4241-4257

[10] Poly(A) signals control both transcriptional termination and initiation between the tandem GAL10 and GAL7 genes of Saccharomyces cerevisiae [J].

Greger, IH ;

Proudfoot, NJ .

EMBO JOURNAL, 1998, 17 (16) :4771-4779